Determination of Cell Doubling Times from the Return-on-Investment Time of Photosynthetic Vesicles Based on Atomic Detail Structural Models [electronic resource]

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Ngôn ngữ: eng

Ký hiệu phân loại: 668.4 Plastics

Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2017

Mô tả vật lý: Size: p. 3787-3797 : , digital, PDF file.

Bộ sưu tập: Metadata

ID: 263314

 Cell doubling times of the purple bacterium <
 em>
 Rhodobacter sphaeroides<
 /em>
  during photosynthetic growth are determined experimentally and computationally as a function of illumination. For this purpose, energy conversion processes in an intracytoplasmic membrane vesicle, the chromatophore, are described based on an atomic detail structural model. The cell doubling time and its illumination dependence are computed in terms of the return-on-investment (ROI) time of the chromatophore, determined computationally from the ATP production rate, and the mass ratio of chromatophores in the cell, determined experimentally from whole cell absorbance spectra. The ROI time is defined as the time it takes to produce enough ATP to pay for the construction of another chromatophore. The ROI time of the low light-growth chromatophore is 4.5?2.6 h for a typical illumination range of 10?100 ?mol photons m<
 sup>
 ?2<
 /sup>
  s<
 sup>
 ?1<
 /sup>
 , respectively, with corresponding cell doubling times of 8.2?3.9 h. Finally, when energy expenditure is considered as a currency, the benefit-to-cost ratio computed for the chromatophore as an energy harvesting device is 2?8 times greater than for photovoltaic and fossil fuel-based energy solutions and the corresponding ROI times are approximately 3?4 orders of magnitude shorter for the chromatophore than for synthetic systems.
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