Glycolysis without pyruvate kinase in Clostridium thermocellum [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 668.5 Perfumes and cosmetics
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2016
• Mô tả vật lý: Size: 28 p. : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 263341
• Chủ đề: 1. 09 biomass fuels; 2. 13c flux analysis; 3. Malate shunt; 4. Malic enzyme; 5. Pyruvate kinase

Tóm tắt

 The metabolism of <
 i>
 Clostridium thermocellum<
 /i>
  is notable in that it assimilates sugar via the EMP pathway but does not possess a pyruvate kinase enzyme. In the wild type organism, there are three proposed pathways for conversion of phosphoenolpyruvate (PEP) to pyruvate, which differ in their cofactor usage. One path uses pyruvate phosphate dikinase (PPDK), another pathway uses the combined activities of PEP carboxykinase (PEPCK) and oxaloacetate decarboxylase (ODC). Yet another pathway, the malate shunt, uses the combined activities of PEPCK, malate dehydrogenase and malic enzyme. First we showed that there is no flux through the ODC pathway by enzyme assay. Flux through the remaining two pathways (PPDK and malate shunt) was determined by dynamic <
 sup>
 13<
 /sup>
 C labeling. In the wild-type strain, the malate shunt accounts for about 33 � 2% of the flux to pyruvate, with the remainder via the PPDK pathway. Deletion of the <
 i>
 ppdk<
 /i>
  gene resulted in a redirection of all pyruvate flux through the malate shunt. Lastly, this provides the first direct evidence of the in-vivo function of the malate shunt.