Transcriptome and proteome analysis of nitrogen starvation responses in Synechocystis 6803 ?glgC, a mutant incapable of glycogen storage [electronic resource]

0 Người đánh giá. Xếp hạng trung bình 0

Tác giả:

Ngôn ngữ: eng

Ký hiệu phân loại: 662.8 Other fuels

Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Energy Efficiency and Renewable Energy ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2016

Mô tả vật lý: Size: p. 64-75 : , digital, PDF file.

Bộ sưu tập: Metadata

ID: 263372

Thêm vào giỏ Liên kết toàn văn

Tóm tắt
Chủ đề

Molecular mechanisms that regulate carbon flux are poorly understood in algae. The 
?glgC
mutant of the cyanobacterium 
Synechocystis
sp. PCC 6803 is incapable of glycogen storage and displays an array of physiological responses under nitrogen starvation that are different from wild-type (WT). These include non-bleaching phenotype and the redirection of photosynthetically fixed carbon towards excreted organic acids (overflow metabolism) without biomass growth. To understand the role of gene/protein expression in these responses, we followed the time course of transcripts by genome-scale microarrays and proteins by shotgun proteomics in 
?glgC
and WT cells upon nitrogen starvation. Compared to WT, the degradation of phycobilisome rod proteins was delayed and attenuated in the mutant, and the core proteins were less degraded
both contributed to the non-bleaching appearance despite the induction of 
nblA
genes, suggesting the presence of a break in regulation of the phycobilisome degradation pathway downstream of 
nblA
induction. The mutant displayed NtcA-mediated transcriptional response to nitrogen starvation, indicating that it is able to sense nitrogen status. Furthermore, some responses to nitrogen starvation appear to be stronger in the mutant, as shown by the increases in transcripts for the transcriptional regulator, 
rre37
, which regulates central carbon metabolism. Accordingly, multiple proteins involved in photosynthesis, central carbon metabolism, and carbon storage and utilization showed lower abundance in the mutant. Furthermore, these results indicate that the transition in the central carbon metabolism from growth to overflow metabolism in 
?glgC
does not require increases in expression of the overflow pathway enzymes
the transition and non-bleaching phenotype are likely regulated instead at the metabolite level.

1. 09 biomass fuels
2. Algae
3. Cyanobacterium
4. Glycogen storage
5. Nitrogen starvation

Tạo bộ sưu tập với mã QR

Transcriptome and proteome analysis of nitrogen starvation responses in <i>Synechocystis</i> 6803 <i>?glgC</i>, a mutant incapable of glycogen storage [electronic resource]