Comparison of nitrogen depletion and repletion on lipid production in yeast and fungal species [electronic resource]

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Ngôn ngữ: eng

Ký hiệu phân loại: 666.9 Masonry adhesives

Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Energy Efficiency and Renewable Energy ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2016

Mô tả vật lý: Size: Article No. 685 : , digital, PDF file.

Bộ sưu tập: Metadata

ID: 263403

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Although it is well known that low nitrogen stimulates lipid accumulation, especially for algae and some oleaginous yeast, few studies have been conducted in fungal species, especially on the impact of different nitrogen deficiency strategies. In this study, we use two promising consolidated bioprocessing (CBP) candidates to examine the impact of two nitrogen deficiency strategies on lipid production, which are the extensively investigated oleaginous yeast 
Yarrowia lipolytica
, and the commercial cellulase producer 
Trichoderma reesei
. We first utilized bioinformatics approaches to reconstruct the fatty acid metabolic pathway and demonstrated the presence of a triacylglycerol (TAG) biosynthesis pathway in 
Trichoderma reesei
. We then examined the lipid production of 
Trichoderma reesei
and 
Y. lipomyces
in different media using two nitrogen deficiency strategies of nitrogen natural repletion and nitrogen depletion through centrifugation. Our results demonstrated that nitrogen depletion was better than nitrogen repletion with about 30% lipid increase for 
Trichoderma reesei
and 
Y. lipomyces
, and could be an option to improve lipid production in both oleaginous yeast and filamentous fungal species. The resulting distinctive lipid composition profiles indicated that the impacts of nitrogen depletion on yeast were different from those for fungal species. Under three types of C/N ratio conditions, C16 and C18 fatty acids were the predominant forms of lipids for both 
Trichoderma reesei
and 
Y. lipolytica
. In addition, while the overall fatty acid methyl ester (FAME) profiles of 
Trichoderma reesei
were similar, the overall FAME profiles of 
Y. lipolytica
observed a shift. The fatty acid metabolic pathway reconstructed in this work supports previous reports of lipid production in 
T. reesei
, and provides a pathway for future omics studies and metabolic engineering efforts. Further investigation to identify the genetic targets responsible for the effect of nitrogen depletion on lipid production improvement will facilitate strain engineering to boost lipid production under more optimal conditions for productivity than those required for nitrogen depletion.

1. 09 biomass fuels
2. 59 basic biological sciences
3. Advanced biofuel
4. Lipids
5. Nitrogen depletion

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