Heterologous expression of family 10 xylanases from Acidothermus cellulolyticus enhances the exoproteome of Caldicellulosiruptor bescii and growth on xylan substrates [electronic resource]

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Ngôn ngữ: eng

Ký hiệu phân loại: 662.1 Fireworks (Pyrotechnics)

Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2016

Mô tả vật lý: Size: Article No. 176 : , digital, PDF file.

Bộ sưu tập: Metadata

ID: 263415

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The ability to deconstruct plant biomass without conventional pretreatment has made members of the genus 
Caldicellulosiruptor
the target of investigation for the consolidated processing of lignocellulosic biomass to biofuels and bioproducts. These Gram-positive bacteria are hyperthermophilic anaerobes and the most thermophilic cellulolytic organisms so far described. They use both C5 and C6 sugars simultaneously and have the ability to grow well on xylan, a major component of plant cell walls. This is an important advantage for their use to efficiently convert biomass at yields sufficient for an industrial process. For commodity chemicals, yield from substrate is perhaps the most important economic factor. In an attempt to improve even further the ability of 
C. bescii
to use xylan, we introduced two xylanases from Acidothermus cellulolyticus. Acel_0180 includes tandem carbohydrate-binding modules (CBM2 and CBM3) located at the C-terminus, one of which, CBM2, is not present in C. bescii. Also, the sequences of Xyn10A and Acel_0180 have very little homology with the GH10 domains present in 
C. bescii
. For these reasons, we selected these xylanases as potential candidates for synergistic interaction with those in the 
C. bescii
exoproteome. As a result, heterologous expression of two xylanases from 
Acidothermus cellulolyticus
in 
Caldicellulosiruptor
bescii resulted in a modest, but significant increase in the activity of the exoproteome of 
C. bescii
on xylan substrates. Even though the increase in extracellular activity was modest, the ability of 
C. bescii
to grow on these substrates was dramatically improved suggesting that the xylan substrate/microbe interaction substantially increased deconstruction over the secreted free enzymes alone. In conclusion, we anticipate that the ability to efficiently use xylan, a major component of plant cell walls for conversion of plant biomass to products of interest, will allow the conversion of renewable, sustainable, and inexpensive plant feedstocks to products at high yields.

1. 09 biomass fuels
2. 37 inorganic, organic, physical, and analytical chemistry
3. Biomass deconstruction
4. Consolidated bioprocessing
5. Xylanase

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Heterologous expression of family 10 xylanases from <i>Acidothermus cellulolyticus</i> enhances the exoproteome of <i>Caldicellulosiruptor bescii</i> and growth on xylan substrates [electronic resource]