Use of <i>De Novo</i> transcriptome libraries to characterize a novel oleaginous marine <i>Chlorella</i> species during the accumulation of triacylglycerols [electronic resource]

 0 Người đánh giá. Xếp hạng trung bình 0

Tác giả:

Ngôn ngữ: eng

Ký hiệu phân loại: 621.48 Nuclear engineering

Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Energy Efficiency and Renewable Energy ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2016

Mô tả vật lý: Size: Article No. e0147527 : , digital, PDF file.

Bộ sưu tập: Metadata

ID: 263520

 Here, marine chlorophytes of the genus <
 i>
 Chlorella<
 /i>
  are unicellular algae capable of accumulating a high proportion of cellular lipids that can be used for biodiesel production. In this study, we examined the broad physiological capabilities of a subtropical strain (C596) of <
 i>
 Chlorella<
 /i>
  sp. ?SAG-211-18? including its heterotrophic growth and tolerance to low salt.We found that the alga replicates more slowly at diluted salt concentrations and can grow on a wide range of carbon substrates in the dark.We then sequenced the RNA of <
 i>
 Chlorella<
 /i>
  strain C596 to elucidate key metabolic genes and investigate the transcriptomic response of the organism when transitioning from a nutrient-replete to a nutrient-deficient condition when neutral lipids accumulate. Specific transcripts encoding for enzymes involved in both starch and lipid biosynthesis, among others, were up-regulated as the cultures transitioned into a lipid-accumulating state whereas photosynthesis-related genes were down-regulated. Transcripts encoding for two of the up-regulated enzymes?a galactoglycerolipid lipase and a diacylglyceride acyltransferase?were also monitored by reverse transcription quantitative polymerase chain reaction assays. The results of these assays confirmed the transcriptome-sequencing data. The present transcriptomic study will assist in the greater understanding, more effective application, and efficient design of <
 i>
 Chlorella<
 /i>
 -based biofuel production systems.
Tạo bộ sưu tập với mã QR

THƯ VIỆN - TRƯỜNG ĐẠI HỌC CÔNG NGHỆ TP.HCM

ĐT: (028) 71010608 | Email: tt.thuvien@hutech.edu.vn

Copyright @2024 THƯ VIỆN HUTECH