Elimination of metabolic pathways to all traditional fermentation products increases ethanol yields in <i>Clostridium thermocellum</i> [electronic resource]

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Tác giả:

Ngôn ngữ: eng

Ký hiệu phân loại: 547.6 Aromatic compounds

Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2015

Mô tả vật lý: Size: p. 49-54 : , digital, PDF file.

Bộ sưu tập: Metadata

ID: 263614

 <
 i>
 Clostridium thermocellum<
 /i>
  has the natural ability to convert cellulose to ethanol, making it a promising candidate for consolidated bioprocessing (CBP) of cellulosic biomass to biofuels. To further improve its CBP capabilities, we study a mutant strain of <
 i>
 C. thermocellum<
 /i>
  that was constructed (strain AG553
  <
 i>
 C. thermocellum ?hpt ?hydG ?ldh ?pfl ?pta-ack<
 /i>
 ) to increase flux to ethanol by removing side product formation. Strain AG553 showed a two- to threefold increase in ethanol yield relative to the wild type on all substrates tested. On defined medium, strain AG553 exceeded 70% of theoretical ethanol yield on lower loadings of the model crystalline cellulose Avicel, effectively eliminating formate, acetate, and lactate production and reducing H<
 sub>
 2<
 /sub>
  production by fivefold. On 5 g/L Avicel, strain AG553 reached an ethanol yield of 63.5% of the theoretical maximum compared with 19.9% by the wild type, and it showed similar yields on pretreated switchgrass and poplar. The elimination of organic acid production suggested that the strain might be capable of growth under higher substrate loadings in the absence of pH control. Final ethanol titer peaked at 73.4 mM in mutant AG553 on 20 g/L Avicel, at which point the pH decreased to a level that does not allow growth of <
 i>
 C. thermocellum<
 /i>
 , likely due to CO<
 sub>
 2<
 /sub>
  accumulation. In comparison, the maximum titer of wild type <
 i>
 C. thermocellum<
 /i>
  was 14.1 mM ethanol on 10 g/L Avicel. In conclusion, with the elimination of the metabolic pathways to all traditional fermentation products other than ethanol, AG553 is the best ethanol-yielding CBP strain to date and will serve as a platform strain for further metabolic engineering for the bioconversion of lignocellulosic biomass.
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