Fermentation of dilute acid pretreated Populus by Clostridium thermocellum, Caldicellulosiruptor bescii, and Caldicellulosiruptor obsidiansis [electronic resource]

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Tác giả: Oak Ridge National Laboratory, United States, United States. Dept. of Energy. Office of Science

Ngôn ngữ: eng

Ký hiệu phân loại: 666.9 Masonry adhesives

Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2015

Mô tả vật lý: Size: p. 1014-1021 : , digital, PDF file.

Bộ sưu tập: Báo, Tạp chí

ID: 263635

Thêm vào giỏ Liên kết toàn văn

Consolidated bioprocessing (CBP), which merges enzyme production, biomass hydrolysis, and fermentation into a single step, has the potential to become an efficient and economic strategy for the bioconversion of lignocellulosic feedstocks to transportation fuels or chemicals. In this study, we evaluated 
Clostridium thermocellum
, 
Caldicellulosiruptor bescii
, and 
Caldicellulosiruptor obsidiansis
, three , thermophilic,cellulolytic, mixed-acid fermenting candidate CBP microorganisms, for their fermentation capabilities using dilute acid pretreated 
Populus
as a model biomass feedstock. Under pH controlled, anaerobic fermentation conditions, each candidate successfully digested a minimum of 75% of the cellulose from dilute acid pretreated 
Populus
, as indicated by an increase in planktonic cells and end-product metabolites and a concurrent decrease in glucan content. 
C. thermocellum
, which employs a cellulosomal approach to biomass degradation, required 120 hours to achieve 75% cellulose utilization. In contrast, the non-cellulosomal, secreted hydrolytic enzyme system of the 
Caldicellulosiruptor
sp. required 300 hours to achieve similar results. End-point fermentation conversions for 
C. thermocellum
, 
C. bescii
, and 
C. obsidiansis
were determined to be 0.29, 0.34, and 0.38 grams of total metabolites per gram of loaded glucan, respectively. This data provide a starting point for future strain engineering efforts that can serve to improve the biomass fermentation capabilities of these three promising candidate CBP platforms.

1. 09 biomass fuels
2. Caldicellulosiruptor
3. Caldicellulosiruptor bescii
4. Clostridia thermocellum
5. Consolidated bioprocessing

Tạo bộ sưu tập với mã QR

Fermentation of dilute acid pretreated <i>Populus</i> by <i>Clostridium thermocellum</i>, <i>Caldicellulosiruptor bescii</i>, and <i>Caldicellulosiruptor obsidiansis</i> [electronic resource]