High-yield hydrogen production from biomass by in vitro metabolic engineering [electronic resource] : Mixed sugars coutilization and kinetic modeling

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Ngôn ngữ: eng

Ký hiệu phân loại: 630.1 Agriculture and related technologies

Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2015

Mô tả vật lý: Size: p. 4964-4969 : , digital, PDF file.

Bộ sưu tập: Metadata

ID: 263683

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The use of hydrogen (H
2
) as a fuel offers enhanced energy conversion efficiency and tremendous potential to decrease greenhouse gas emissions, but producing it in a distributed, carbon-neutral, low-cost manner requires new technologies. Herein we demonstrate the complete conversion of glucose and xylose from plant biomass to H
2
and CO
2
based on an in vitro synthetic enzymatic pathway. Glucose and xylose were simultaneously converted to H
2
with a yield of two H
2
per carbon, the maximum possible yield. Parameters of a nonlinear kinetic model were fitted with experimental data using a genetic algorithm, and a global sensitivity analysis was used to identify the enzymes that have the greatest impact on reaction rate and yield. After optimizing enzyme loadings using this model, volumetric H
2
productivity was increased 3-fold to 32 mmol H
2
?L
?1
?h
?1
. The productivity was further enhanced to 54 mmol H
2
?L
?1
?h
?1
by increasing reaction temperature, substrate, and enzyme concentrations?an increase of 67-fold compared with the initial studies using this method. The production of hydrogen from locally produced biomass is a promising means to achieve global green energy production.

1. 08 hydrogen
2. 09 biomass fuels
3. 32 energy conservation, consumption, and utilization
4. Biomass
5. Hydrogen

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