Identification of MicroRNAs and transcript targets in <i>Camelina sativa</i> by deep sequencing and computational methods [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 631.531 Propagation from seeds (Sowing)
• Thông tin xuất bản: Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2015
• Mô tả vật lý: Size: Article No. 0121542 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 263684
• Chủ đề: 1. 09 biomass fuels; 2. 59 basic biological sciences

Tóm tắt

 <
 i>
 Camelina sativa<
 /i>
  is an annual oilseed crop that is under intensive development for renewable resources of biofuels and industrial oils. MicroRNAs, or miRNAs, are endogenously encoded small RNAs that play key roles in diverse plant biological processes. Here, we conducted deep sequencing on small RNA libraries prepared from camelina leaves, flower buds and two stages of developing seeds corresponding to initial and peak storage products accumulation. Computational analyses identified 207 known miRNAs belonging to 63 families, as well as 5 novel miRNAs. These miRNAs, especially members of the miRNA families, varied greatly in different tissues and developmental stages. The predicted miRNA target genes are involved in a broad range of physiological functions including lipid metabolism. This report is the first step toward elucidating roles of miRNAs in <
 i>
 C. sativa<
 /i>
  and will provide additional tools to improve this oilseed crop for biofuels and biomaterials.