Production of deuterated switchgrass by hydroponic cultivation [electronic resource]

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Tác giả:

Ngôn ngữ: eng

Ký hiệu phân loại: 630.966 Agriculture and related technologies

Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2015

Mô tả vật lý: Size: p. 215-222 : , digital, PDF file.

Bộ sưu tập: Metadata

ID: 263692

 Deuterium enrichment of biological materials can potential enable expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls. Two key advances have been made that facilitate cultivation of switchgrass, an important forage and biofuel crop, for controlled isotopic enrichment: (1) perfusion system with individual chambers and (2) hydroponic growth from tiller cuttings. Plants were grown and maintained for several months with periodic harvest. Photosynthetic activity was monitored by measurement of CO<
 sub>
 2<
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  in outflow from the growth chambers. Plant morphology and composition appeared normal compared to matched controls grown with H<
 sub>
 2<
 /sub>
 O. Using this improved method, gram quantities of switchgrass leaves and stems were produced by continuous hydroponic cultivation using growth medium consisting of basal mineral salts in 50% D<
 sub>
 2<
 /sub>
 O. Deuterium incorporation was confirmed by detection of the O-D and C-D stretching peaks with FTIR and quantified by <
 sup>
 1<
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 H- and <
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 H-NMR. Lastly, this capability to produce deuterated lignocellulosic biomass under controlled conditions will enhance investigation of cell wall structure and its deconstruction by neutron scattering and NMR techniques.
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