Preservation of H ₂ production activity in nanoporous latex coatings of <i>Rhodopseudomonas palustris</i> CGA009 during dry storage at ambient temperatures [electronic resource] : Preservation of <i>R.palustris</i> latex coatings

• Ngôn ngữ: eng
• Ký hiệu phân loại: 621.4 Prime movers and heat engineering
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Energy Efficiency and Renewable Energy ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2013
• Mô tả vật lý: Size: p. 515-525 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 263868
• Chủ đề: 1. 09 biomass fuels

Tóm tắt

 To assess the applicability of latex cell coatings as an ?off-the-shelf? biocatalyst, the effect of osmoprotectants, temperature, humidity and O<
 sub>
 2<
 /sub>
  on preservation of H<
 sub>
 2<
 /sub>
  production in Rhodopseudomonas palustris coatings was evaluated. Immediately following latex coating coalescence (24 h) and for up to 2 weeks of dry storage, rehydrated coatings containing different osmoprotectants displayed similar rates of H<
 sub>
 2<
 /sub>
  production. Beyond 2 weeks of storage, sorbitol-treated coatings lost all H<
 sub>
 2<
 /sub>
  production activity, whereas considerable H<
 sub>
 2<
 /sub>
  production was still detected in sucrose- and trehalose-stabilized coatings. We stored the coatings at a relative humidity level which significantly impacts the recovery and subsequent rates of H<
 sub>
 2<
 /sub>
  production. After 4 weeks storage under air at 60% humidity, coatings produced only trace amounts of H<
 sub>
 2<
 /sub>
  (0?0.1% headspace accumulation), whereas those stored at <
  5% humidity retained 27?53% of their H<
 sub>
 2<
 /sub>
  production activity after 8 weeks of storage. Furthermore, when stored in argon at <
  5% humidity and room temperature, R. palustris coatings retained full H<
 sub>
 2<
 /sub>
  production activity for 3 months, implicating oxidative damage as a key factor limiting coating storage. Ultimately, the results demonstrate that biocatalytic latex coatings are an attractive cell immobilization platform for preservation of bioactivity in the dry state.