Biologically Diverse Genetic Engineering Platform [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 621.47 Solar-energy engineering
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2020
• Mô tả vật lý: Size: 3 p. : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 264113
• Chủ đề: 1. 09 biomass fuels; 2. 59 basic biological sciences

Tóm tắt

This project focused on methods to genetically edit arbitrary prokaryotes to convert non-agricultural biomass to industrially relevant chemicals at scale and at a price point that would be competitive with petroleum. Our target chemical for this demonstration was succinic acid, a polymer precursor that could theoretically be produced at scale from biomass. We demonstrated genetic control over a selected bacterial type strain: we can make permanent and directed changes to its genome. After thoroughly characterizing its behavior, and the circumstances under which it produced the most succinic acid, the researchers found a wild-type isolate that produces at least eight times more succinic acid than the type strain.