Expression and Characterization of Chimeric CBHI in Yeasts and the Effects of CBHI Fusion to EGII [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 662.7 Coke and charcoal
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Energy Efficiency and Renewable Energy ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2019
• Mô tả vật lý: Size: 944 KB : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 264292
• Chủ đề: 1. 09 biomass fuels; 2. Cbhi; 3. Cellobiohydrolasei; 4. Saccharomyces cerevisiae; 5. Yeasts

Tóm tắt

The low secretion levels of cellobiohydrolaseI (CBHI) in yeasts is one of the key barriers preventing yeast from directly degrading and utilizing lignocellulose. To overcome this barrier, we have explored the approach of genetically linking an easily secreted protein to CBHI, with CBHI being the last to be folded. The Trichoderma reesei eg2 (TrEGII) gene was selected as the leading gene due to its previously demonstrated outstanding secretion in yeast. To comprehensively characterize the effects of this fusion protein, we tested this hypothesis in three industrially relevant yeasts: Saccharomyces cerevisiae, Yarrowia lipolytica, and Lipomyces starkeyi.