Quantification of triglyceride content in oleaginous materials using thermo-gravimetry [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 662.8 Other fuels
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Energy Efficiency and Renewable Energy ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2017
• Mô tả vật lý: Size: p. 232-237 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 264425
• Chủ đề: 1. 09 biomass fuels; 2. 60 applied life sciences; 3. Lipid analysis; 4. Thermo-gravimetry; 5. Triglyceride quantification

Tóm tắt

Laboratory analytical methods for quantification of triglyceride content in oleaginous biomass samples, especially microalgae, require toxic chemicals and/or organic solvents and involve multiple steps. We describe a simple triglyceride quantification method that uses thermo-gravimetry. This method is based on the observation that triglycerides undergo near-complete volatilization/degradation over a narrow temperature interval with a derivative weight loss peak at 420 �C when heated in an inert atmosphere. Degradation of the other constituents of oleaginous biomass (protein and carbohydrates) is largely complete after prolonged exposure of samples at 320 �C. Based on these observations, the triglyceride content of oleaginous biomass was estimated by using the following two-step process. In Step 1, samples were heated to 320 �C and kept isothermal at this temperature for 15 min. In Step 2, samples were heated from 320 �C to 420 �C and then kept isothermal at 420 �C for 15 min. The results show that mass loss in step 2 correlated well with triglyceride content estimates obtained from conventional techniques for diverse microalgae and oilseed samples.