MBTH [electronic resource] : A novel approach to rapid, spectrophotometric quantitation of total algal carbohydrates

• Ngôn ngữ: eng
• Ký hiệu phân loại: 333.79 Energy
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Energy Efficiency and Renewable Energy ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2016
• Mô tả vật lý: Size: p. 90-93 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 264558
• Chủ đề: 1. 09 biomass fuels; 2. 59 basic biological sciences; 3. Algae; 4. Carbohydrates; 5. Spectrophotometric

Tóm tắt

 A high-throughput and robust application of the 3-methyl-2-benzothiazolinone hydrazone (MBTH) method was developed for carbohydrate determination in microalgae. The traditional phenol-sulfuric acid method to quantify carbohydrates is strongly affected by algal biochemical components and exhibits a highly variable response to microalgal monosaccharides. We present a novel use of the MBTH method to accurately quantify carbohydrates in hydrolyzate after acid hydrolysis of algal biomass, without a need for neutralization. As a result, the MBTH method demonstrated consistent and sensitive quantitation of algae-specific monosaccharides down to 5 ug mL<
 sup>
 -1<
 /sup>
  without interference from other algae acidic hydrolyzate components.