This product builds on previous efforts, namely the 2005 Billion-Ton Study (BTS) and the 2011 U.S. Billion-Ton Update (BT2).With each report, greater perspective is gained on the potential of biomass resources to contribute to a national energy strategy. Similarly, each successive report introduces new questions regarding commercialization challenges. BTS quantified the broad biophysical potential of biomass nationally, and BT2 elucidated the potential economic availability of these resources. These reports clearly established the potential availability of up to one billion tons of biomass resources nationally. However, many questions remain, including but not limited to crop yields, climate change impacts, logistical operations, and systems integration across production, harvest, and conversion. The present report aims to address many of these questions through empirically modeled energy crop yields, scenario analysis of resources delivered to biorefineries, and the addition of new feedstocks. Volume 2 of the 2016 Billion-Ton Report is expected to be released by the end of 2016. It seeks to evaluate environmental sustainability indicators of select scenarios from volume 1 and potential climate change impacts on future supplies.