Applications of Carboxylic Acid Reductases in Oleaginous Microbes [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 660.6 Biotechnology
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Energy Efficiency and Renewable Energy ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2016
• Mô tả vật lý: Size: 807 KB : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 264650
• Chủ đề: 1. 09 biomass fuels; 2. Biomass; 3. Carboxylic acid reductase; 4. Fatty acids; 5. Fuels

Tóm tắt

Carboxylic acid reductases (CARs) are recently emerging reductive enzymes for the direct production of aldehydes from biologically-produced carboxylic acids. Recent work has demonstrated that these powerful enzymes are able to reduce a very broad range of volatile- to long-chain fatty acids as well as aromatic acids. Here, we express four CAR enzymes from different fungal origins to test their activity against fatty acids commonly produced in oleaginous microbes. These in vitro results will inform metabolic engineering strategies to conduct mild biological reduction of carboxylic acids in situ, which is conventionally done via hydrotreating catalysis at high temperatures and hydrogen pressures.