Value Added Products from Renewable Biofuels [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 662.7 Coke and charcoal
• Thông tin xuất bản: Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2014
• Mô tả vật lý: Size: 6 p. : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 264915
• Chủ đề: 1. 09 biomass fuels

Tóm tắt

 Cellulosic ethanol is an emerging biofuel that will make strong contributions to American domestic energy needs. In the US midwest the standard method for pretreatment of biomass uses hot acid to deconstruct lignocellulose. While other methods work, they are not in common use. Therefore it is necessary to work within this context to achieve process improvements and reductions in biofuel cost. Technology underlying this process could supplement and even replace commodity enzymes with engineered microbes to convert biomass-derived lignocellulose feedstocks into biofuels and valueadded chemicals. The approach that was used here was based on consolidated bioprocessing. Thermoacidophilic microbes belonging to the Domain Archaea were evaluated and modfied to promote deconvolution and saccharification of lignocellulose. Biomass pretreatment (hot acid) was combined with fermentation using an extremely thermoacidophilic microbial platform. The identity and fate of released sugars was controlled using metabolic blocks combined with added biochemical traits where needed. LC/MS analysis supported through the newly established Nebraska Bioenergy Facility provided general support for bioenergy researchers at the University of Nebraska. The primary project strategy was to use microbes that naturally flourish in hot acid (thermoacidophiles) with conventional biomass pretreatment that uses hot acid. The specific objectives were: to screen thermoacidophilic taxa for the ability to deconvolute lignocellulose and depolymerize associated carbohydrates
  evaluate and respond to formation of ?inhibitors? that arose during incubation of lignocellulose under heated acidic conditions
  identify and engineer ?sugar flux channeling and catabolic blocks? that redirect metabolic pathways to maximize sugar concentrations
  expand the hydrolytic capacity of extremely thermoacidophilic microbes through the addition of deconvolution traits
  and establish the Nebraska Bioenergy Facility (NBF) at the University of Nebraska-Lincoln.