Unveiling the biological variation of lymphocyte subset counts: Insights from a full-spectrum flow cytometer and the BD Multitest

• Tác giả: Zhongli Du, Xiaoran Feng, Kai Guo, Chenbin Li, Hong Lu, Yating Ma, Mingting Peng, Lei Xu
• Ngôn ngữ: eng
• Thông tin xuất bản: Netherlands : Clinica chimica acta; international journal of clinical chemistry , 2025
• Bộ sưu tập: NCBI
• ID: 268780

Tóm tắt

BACKGROUND AND AIMS: To estimate the biological variation (BV) for lymphocyte subset counts in healthy adults based on full-spectrum flow cytometry (FS-FCM) and the most commonly used BD Multitest MATERIALS AND METHODS: The study was designed according to the BV Data Critical Appraisal Checklist (BIVAC). Peripheral blood samples were collected from 60 healthy adults every two weeks for a period of 20 weeks (10 samples from each subject). Lymphocyte subsets were quantified using FS-FCM and the kit mentioned above. Bayesian models were used to analyze within-subject BV (CV RESULTS: The CV CONCLUSIONS: To the best of our knowledge, this study is the first to estimate the BV of lymphocyte subset counts based on FS-FCM and the clinically commonly used BD Multitest