The structure and anti-SARS-CoV-2 activity of sulfated polysaccharides (Mzpt) obtained in high yield (60 %) from tetrasporophytes of Mazzaella parksii were studied. Stepwise fractionation with KCl showed that Mzpt consisted of eight (MzptF1-MzptF8) carrageenans fractions, differing in structure and molecular weight. The yield of non-gelling MzptF8 was 58.1 % of the original Mzpt. According to IR and NMR spectroscopies, gelling MzptF1 and MzptF2 were mainly kappa/iota/nu-carrageenans. MzptF7 included mainly lambda-carrageenan and in smaller quantities kappa-, iota-, mu- and nu-carrageenans. MzptF8 had a complex composition and included gamma-carrageenan, unsulfated carrageenan, probably, delta-carrageenan and also structural elements of xi-, psi- and omicron-carrageenans. According to atomic force microscopy data, MzptF8 involved several polymer chains associated with each other in a disordered structure, in contrast to MzptF2, which formed three-dimensional networks. Unlike ribavirin and remdesivir, Mzpt was not cytotoxic to Vero E6 cells at concentrations >
2000 μg mL