Synthesis of chiral alcohol (S)-CHBE by co-immobilization of double enzymes based on organic-inorganic hybrid nanoflower.

• Tác giả: Peng Chen, Fenghuan Wang, Yadong Wang, Xinpeng Yang
• Ngôn ngữ: eng
• Ký hiệu phân loại: 378.242 Thesis and dissertation requirements
• Thông tin xuất bản: Netherlands : International journal of biological macromolecules , 2025
• Bộ sưu tập: NCBI
• ID: 270544

Tóm tắt

The chiral alcohols (S)-4-chloro-3-hydroxy-butyric acid ethyl ester ((S)-CHBE) is a critical intermediate in the synthesis of various active pharmaceutical ingredients. This study presents the first investigation of the efficient production of (S)-CHBE using organic-inorganic hybrid nanoflowers (GDH-CR@HNFs) for the co-immobilization of glucose dehydrogenase (BsGDH) and carbonyl reductase (BsCR). By optimizing immobilization conditions, we significantly enhanced the catalytic activity and immobilization efficiency of the hybrid nanoflowers. The GDH-CR@HNFs exhibited superior catalytic performance compared to the free dual enzyme system, demonstrating a higher affinity for the substrate COBE (47-fold lower K