Thử nghiệm sản xuất giống cá trê vàng (Clarias macrocephalus) bằng các loại kích dục tố khác nhau tại Trường Đại học Bạc Liêu Experiment on Broadhead catfish (Clarias macrocephalus) artificial breeding using different types of hormones in Bac Lieu Univer

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Tác giả: Trần Thị Bích Như, Nguyễn Thị Hồng Vân, Lê Hoàng Vũ

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học Trường Đại học Bạc Liêu, 2024

Mô tả vật lý: tr.2

Bộ sưu tập: Metadata

ID: 272260

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The research investigated the impact of various hormonal stimulants on the maturation of broadhead catfish (Clarias macrocephalus). Three treatments were utilized in the study: NT1 (80µg LRHa3 + 0.8mg DOM), NT2 (4,000IU HCG), and NT3 (2,000IU HCG + 40µg LRHa3 + 0.4mg DOM)/kg of female fish. Each treatment was performed in triplicate with a randomized design. Female fish were injected with 2 doses of hormone, whereas males were injected with a single dose which was ½ of the total injected dose of the female fish and at the same time as the female's 2nd dose. The spawning time fell within the interval of 8:05 - 9:45 hours after injection, in environmental conditions that included temperatures variable from 28.5 - 29.3°C, dissolved oxygen levels of 5ppm, and pH fluctuations of 8.27 - 8.43. The range of the spawning rate was 83.33 % - 100 % with NT3 having the highest fertilization rate at 81.79 ± 4.88 %, NT1 at 79.26 ± 4.49 %, and NT2 at 71.49 ± 11.76 %. The hatching rate proved highest at NT1 (79.70 ± 8.03%), followed by NT2 (72.88 ± 13.55%), and lowest at NT3 (42.09 ± 17.40%), with significant differences observed compared to NT1 and NT2 (p<
0.05). Survival rates were similar among treatments (p>
0.05), with NT1 having the greatest rate (83.70 ± 12.92%), followed by NT2 (82.55 ± 7.81%) and NT3 having the lowest (71.46 ± 4.23%). The findings suggest that using 80µg LRHa3 + 0.8mg DOM/kg of female effectively stimulates the reproduction of broadhead catfish Clarias macrocephalus. Nghiên cứu sản xuất giống cá trê vàng bằng các loại kích dục tố khác nhau được thực hiện với 3 nghiệm thức: NT1 (LRHa3 80µg + DOM 0,8mg), NT2 (HCG 4.000UI), NT3 (HCG 2.000UI + LRHa3 40µg + DOM 0,4mg)/kg cá cái. Mỗi NT được lặp lại 3 lần và bố trí hoàn toàn ngẫu nhiên. Cá cái được tiêm 2 liều, cá đực được tiêm 1 liều duy nhất cùng thời điểm với liều quyết định của cá cái và liều bằng ½ tổng liều tiêm của cá cái. Trong điều kiện môi trường nhiệt độ từ 28,5 – 29,30C, DO 5ppm và pH 8,27 – 8,43, thời gian hiệu ứng thuốc dao động từ 8h05’ – 9h45’, tỷ lệ sinh sản đạt từ 83,33 – 100%, tỷ lệ thụ tinh trung bình cao nhất ở NT3 là 81,79 ± 4,88%, tiếp đến là NT1 79,26 ± 4,49% và thấp nhất ở NT2 là 71,49 ± 11,76%, tỷ lệ nở cao nhất ở NT1 là 79,70 ± 8,03%, tiếp đến là NT2 là 72,88 ± 13,55% và thấp nhất ở NT3 là 42,09 ± 17,40% và khác biệt có ý nghĩa thống kê so với NT1 và NT2 (p<
0,05). Kết quả tỷ lệ sống ở các NT khác biệt không có ý nghĩa thống kê (p<
0,05), tỷ lệ sống cao nhất ở NT1 là 83,70 ± 12,92%, ở NT2 là 82,55 ± 7,81% và thấp nhất ở NT3 là 71,46 ± 4,23%. Kết quả cho thấy sử dụng 80µg LRHa3 + 0,8mg DOM/kg cá cái kích thích sinh sản cá trê vàng đạt hiệu quả cao.

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