High-resolution imaging of cellular proteins : methods and protocols

• Tác giả: Thomas Schikorski, Steven D Schwartzbach, Omar Skalli
• Ngôn ngữ: eng
• ISBN-10: 1493963503
• ISBN-13: 978-1493963508
• Ký hiệu phân loại: 570.2825 Miscellany
• Mô tả vật lý: xiii, 366 pages : , illustrations (some color) ; , 27 cm
• Bộ sưu tập: Khoa học tự nhiên
• ID: 278467
• Chủ đề: 1. Electron microscopy; 2. Electron microscopy; 3. Proteins--Structure; 4. Proteins--Structure; 5. Image Enhancement--methods; 6. Proteins--ultrastructure; 7. Cells--cytology; 8. Microscopy, Confocal--methods; 9. Microscopy, Electron--methods; 10. Structure-Activity Relationship

Includes bibliographical references and index

Tóm tắt

"This volume presents authoritative and cutting-edge methods and protocols focusing on three tool boxes covering the increasingly diverse methodologies used to image selected proteins and to investigate their function by light and electron microscopy. The first tool box includes the development of a wide range of molecular and immunological probes to target specific proteins. The second details the use of these probes for high resolution fluorescence microscopy and the third focuses on applications for transmission and scanning electron microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls thus ensuring successful results in the further study of this vital field."-- Back cover