As the vital the biomacromolecule in eukaryotic cells, tubulin protein is essential for preserving cell shape, facilitating cell division, and cell viability. Tubulin has been approved as promising target for anticancer, and antifungal therapy. However, there are still many gaps in tubulin-targeted fungicidal discovery. To expand the structural diversity of benzimidazoles and achieve the distinct interaction model, a series of novel benzimidazole hydrazone derivatives were therefore synthesized. Antifungal results showed that compound A