This study successfully synthesised and characterised composites combining chitosan (CH), carboxymethyl cellulose (CMC), and various flavonoids (Fla). This innovative approach demonstrates the potential for developing functional materials with antioxidant and food preservation properties. The composites CH-Fla-CMC (1-5) was characterised using advanced techniques such as FT-IR, UV-Vis, XRD, SEM, TEM, and TGA, providing robust data on their structural, morphological, and thermal properties. CH-connected CMC has been used to prevent many diseases, based on the findings of this study. Therefore, dietary flavonoids (Fla = 1. 3-Hydroxyflavone
2. rhamnetin
3. natsudaidain
4. isorhamnetin
5. myricetin) was used to prepare the composites in this study. Dietary flavonoids play an important role in the prevention of degenerative diseases. In addition, oxygen permeability (OP), water solubility (WS), and moisture content (MS) were analysed. The synthesised composites were screened for antioxidant and cytotoxic activities. Multiple antioxidant assays (DPPH, H₂O₂, NO, ABTS