NGHIÊN CỨU BIẾN NẠP VÀ XÁC ĐỊNH SỰ CÓ MẶT CỦA GEN MÃ HÓA YẾU TỐ PHIÊN MÃ ZmbZIP72 Ở CÂY NGÔ THẾ HỆ T0

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Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2020

Mô tả vật lý: tr.50-56

Bộ sưu tập: Báo, Tạp chí

ID: 292604

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Các nghiên cứu đã cho thấy gen ZmbZIP72 có tầm quan trọng đáng kể tới sự chống chịu hạn của thực vật cũng như với cây ngô. Trong nghiên cứu này, vector biểu hiện thực vật mang gen ZmbZIP72 dưới sự điều khiển của promoter cảm ứng stress RD29A được chuyển vào cây ngô. Thí nghiệm chuyển gen được tiến hành trong 2 vụ là thu đông 2016 và 2017. Khi các cây ngô chuyển gen phát triển hoàn chỉnh trong phòng thí nghiệm thì được trồng ra nhà lưới để cây sinh trưởng phát triển và tiến hành tự thụ phấn cho cây. Phương pháp PCR với cặp mồi nhân gen chỉ thị Hygromycin và nhân gen ZmbZIP72 được sử dụng để kiểm tra sự có mặt của gen chuyển trên các cây ngô. Vụ thu đông 2016, nhóm tác giả thu được 44 cây chuyển gen sống sót trong nhà lưới và có 2 cây dương tính với cặp mồi nhân gen Hyg. Với vụ thu đông 2017, có 140 cây chuyển gen sống sót và 17 cây dương tính với cặp mồi nhân gen ZmbZIP72 và gen Hyg. Như vậy, trong nghiên cứu này, nhóm tác giả đã thu được các cây chuyển gen T0 và sẽ đánh giá các thế hệ tiếp theo trong những vụ gieo hạt sau.Studies have shown that the ZmbZIP72 gene is of significant importance to the drought tolerance of plants as well as to maize. In this study, the expression vector of plants carrying ZmbZIP72 gene under the control of RD29A stress-induced promoter was transferred into maize. Transgenic experiments were conducted in autumn-winter crops 2016 and 2017. The transgenic maize plants were fully developed in the tissue culture laboratory then grown up in the greenhouse for self - pollination. Then, PCR to amplify ZmbZIP72 and Hyg genes was used to test the presence of transgenes in maize plants. The results shown that, in autumn-winter of 2016, 44 transgenic plants survived in the greenhouse therein 2/44 were positive with Hyggene. In the autumn-winter crop of 2017, there were 140 transgenic plants surviving with 17 positive plants which were verified with ZmbZIP72 primers and Hyg primers. In conclusion, the authors have obtained T0 transgenic plants, the next generations will be genotype evaluated in following season crop.

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