STUDY OF ADVENTITIOUS ROOT FORMATION DERIVED FROM NODE OF Morinda officinalis How. CULTURED IN VITRO

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Tác giả: Thanh Loc Le, Thi Kim Mong Ngo, Thi Quy Co Nguyen, Trong Tuan Tran, Thi Huong Trinh

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học Công nghệ và Thực phẩm (Tên mới: Tạp chí Khoa học Đại học Công Thương), 2020

Mô tả vật lý: tr.72

Bộ sưu tập: Metadata

ID: 294040

Thêm vào giỏ Liên kết toàn văn

Cây ba kích là một cây dược liệu quý và có giá trị kinh tế cao của Việt Nam. Trong những năm gần đây, cùng với xu hướng nhân giống và bảo tồn các loài cây dược liệu thì nuôi cấy tạo sinh khối rễ cây ba kích cũng đang được quan tâm. Trong nghiên cứu này, vai trò của IBA (indole-3-butyric acid) và phương pháp đặt mẫu trong sự cảm ứng phát sinh rễ bất định đã được nghiên cứu. Kết quả nghiên cứu cho thấy, mẫu đốt thân được đặt đứng và nuôi cấy trên môi trường SH có bổ sung 2 mg/L IBA thích hợp cho sự cảm ứng tạo rễ bất định cây ba kích in vitro, với tỷ lệ mẫu tạo rễ đạt 100% và số rễ đạt 13,46 rễ/mẫu. Trạng thái môi trường nuôi cấy và nồng độ sucrose cũng đóng vai trò quan trọng đối với sự tăng sinh rễ bất định cây ba kích. Đối với quá trình tăng sinh rễ bất định, các mẫu được nuôi cấy trên môi trường SH có bổ sung 8 g/L agar có hệ số tăng sinh cao hơn so với các mẫu được nuôi cấy trong môi trường lỏng lắc (không bổ sung agar). Trong thí nghiệm nghiên cứu về nồng độ đường, môi trường có bổ sung 45 g/L sucrose thích hợp cho sự tăng sinh khối rễ bất định. Khối lượng mẫu tươi thu được sau 6 tuần nuôi cấy tăng gấp 6 lần so với khối lượng được sử dụng ban đầu (1,2 g). Kết quả đạt được của nghiên cứu là tiền đề cho quy trình nuôi cấy thu nhận sinh khối rễ ba kích ở quy mô lớn, nhằm cung cấp nguồn nguyên liệu ba kích một cách chủ động cho ngành công nghiệp dược.Morinda officinalis is a precious medicinal plant with high economic value in Vietnam. In recent years, along with the tendency of breeding and preserving medicinal plants, the cultivation of biomass roots has been receiving attention. In this study, the role of IBA (indole-3-butyric acid), method of placing node on root induction were investigated. The results showed that SH (Schenk & Hildebrandt) medium supplemented with 2 mg/L IBA was suitable for adventitious root induction of Morinda officinalis cultured in vitro. The node explant placed vertically obtained the higher ratio of root formation (100%) and numbers of root (13.46 roots/sample) than node explant placed horizontally. The state of medium culture and the concentration of sucrose also play an important role in the proliferation of adventitious root. The results indicated that explants cultured on solid SH medium (agar 8.0 g/L) had higher growth rates than explants cultured on shaking liquid medium (without agar). In the experiment using sucrose, the medium supplemented with 45 g/L sucrose was suitable for adventitious root proliferation. The fresh weight of adventitious root achieved the highest value which was six times higher than initial used weight (1,2 g). The results of the study can be referred for the process of culturing Morinda officinalis root biomass at large scale, providing materials for the pharmaceutical industry.

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