OPTIMIZATION OF EXTRACTION OF BETALAIN FROM RED BEETROOT (Beta vulgaris var. rubra (L.) Moq)

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Tác giả: Thi Ngoc Nhon Hoang, Thi Thanh Hang Nguyen

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học Công nghệ và Thực phẩm (Tên mới: Tạp chí Khoa học Đại học Công Thương), 2020

Mô tả vật lý: tr.93

Bộ sưu tập: Metadata

ID: 294095

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Betalain is a pigment found in plants, can be red-violet (betacyanin) or yellow (betaxanthin), soluble in water, a compound widely used in chemistry, medicine and pharmacy with the chemical stability in a wide pH range. Betalain is an antioxidant with antiviral, anti-inflammatory properties. They are found in high levels in red beetroot and are a food coloring additive. The aim of this work was to study factors affecting the extraction of betalain from red beetroots such as solvent type, solvent concentration, material-solvent ratio, temperature and time. The results showed that 2.955 mg/g of dry matter was gained in the suitable extraction conditions such as ethanol 20%, material-to-solvent ratio 1/25 (w/v) at 40 °C for 180 minutes. Optimization of extraction of betalains from red beetroot using the response surface method (RSM) with three-factor central composite rotatable design (CCRD) showed the optimized parameters including material-to-solvent ratio 1:22.96 (w/v), the temperature at 47.71 °C, the period of 183.65 minutes gave the highest content of betalain (3.467 mg/g dry matter).Betalain là sắc tố trong thực vật có màu từ đỏ-tím (betacyanin) đến màu vàng (betaxanthin), hòa tan trong nước, là hợp chất được sử dụng nhiều trong hóa học, y học và dược với tính ổn định về mặt hóa học trong khoảng pH rộng. Betalain có tính oxy hóa, có khả năng kháng virus, kháng viêm. Chúng được tìm thấy với hàm lượng cao trong củ dền và là một phụ gia thực phẩm tạo màu. Mục tiêu của nghiên cứu này là khảo sát các yếu tố ảnh hưởng dung môi: loại dung môi, nồng độ dung môi, tỷ lệ nguyên liệu/dung môi, nhiệt độ, thời gian đến khả năng trích ly betalain từ củ dền (Beta vulgaris var. rubra (L.) Moq). Kết quả thu được hàm lượng betalain 2,955 mg/g chất khô ở các điều kiện: dung môi ethanol 20%, tỷ lệ nguyên liệu/dung môi 1/25 (w/v) tại nhiệt độ 40 °C trong thời gian 180 phút. Tối ưu hóa quá trình trích ly betalain từ củ dền theo phương pháp đáp ứng bề mặt (RSM) với 3 yếu tố theo mô hình CCRD cho thấy, với tỷ lệ nguyên liệu/dung môi 1:22,96 (w/v), nhiệt độ 47,71 °C, thời gian 183,654 phút thu được hàm lượng betalain tối ưu là 3,467 mg/g chất khô.

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