Miliusa velutina leaf contains a promising source of bioactive compounds since it has beentraditionally used for the treatment of various diseases. The present study aimed at evaluating the effectof different solvents on phytochemical constituents, antioxidant and in vitro anti-inflammatory activitiesof Miliusa velutina leaves extracts. Aqueous solvent was identified as the most effective one for theextraction, resulting as the highest content of polyphenol (13.85±0.13 mg GAE/g extract) and flavonoid(267.34±4.93 mg QE/g extract). In addition, the study was conducted to evaluate the antioxidant andanti-inflammatory activities of Miliusa velutina leaves extracts. The antioxidant activity was determinedusing nitric oxide radical inhibition assay. In vitro anti-inflammatory activity was evaluated usingalbumin denaturation method. The results showed that the aqueous extract exhibited high capacity ofantioxidant (EC50 value of 157.08±2.90 μg/mL) and in vitro anti-inflammatory activity (i.e., albumindenaturation: IC50=21.13±0.95 μg/mL). The antioxidant activity of the aqueous extract was found to be4.63-fold higher than vitamin C, and the anti-inflammatory activity of Miliusa velutina extracts wascomparable to diclofenac and prednisolon. Based on the obtained results, it can be concluded thatMiliusa velutina leaves extracts displayed anti-inflammatory and antioxidant activity.Keywords: Anti-inflammatory, antioxidant, diclofenac, Miliusa velutina, nitric oxide, prednisolone