Introduction: Leachate is a noticeable pollution problem because it contains a considerable amount of persistent organic pollutants (POPs). If leachate isn't treated thoroughly, its leak will negatively affect the environment. Therefore, appropriate treatment technologies are required to remove them. Bio-Electro-Fenton (BEF) is a new method using microorganisms such as electrolytes to convert chemical energy into electricity to help create H2O2 support advanced oxidation process (AOPs). Realizing the potentials that BEF brings, this study applies BEF to assess the effectiveness of leachate treatment at Da Phuoc landfill (operation period >
12 years), Ho Chi Minh City, which to save costs and energy for Fenton process. Methods: The BEF pilot scale model (30 x 10 x 10 cm) is divided by a proton exchange membrane (PEM) (Nafion®112) into two chambers (anode andcathode). Cathode chamber used a graphite electrode, the anode chamber used a carbon fabric electrode. The experiments aimed to determine the optimal conditions of parameters affecting the BEF system by determining the efficiency of COD removal and BOD5/COD ratio in leachate. Results: At optimal conditions of the model including pH 3, [Fe2+ ] = 4g/L, current intensity = 1A, reaction time 60 minutes and airflow = 12 L/min, as a result COD was reduced by 68.2 ± 1.04 % from 4950 ±14 mgO2/L to 1574.1 ± 51.4 mgO2/L, the ratio of BOD5/COD = 0.1 Conclusion: The study result showed thatBio-electro-Fenton process is effective for wastewater with high concentrations of pollutant and difficult to treat as leachate suggesting that the appropriate method for pre-treatment processes support the thorough elimination of pollutants.