NHÂN GIỐNG IN VITRO LAN MOKARA THÔNG QUA PROTOCORM-LIKE BODY TỪ MÔ LÁ

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Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2020

Mô tả vật lý: tr.280-285

Bộ sưu tập: Báo, Tạp chí

ID: 302122

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Mokara is a hybrid orchid derived from three genetically different genera Arachnis, Ascocentrum and Vanda. This hybrid orchid is one of the important flower cuttings and is widely grown in many countries such as Singapore, Indonesia, Thailand... and Vietnam. This research aims to micropropagate Mokara orchid from leaf tissue through PLB generation. The results of this study showed that MS medium supplemented with 2.0 mg l-1 2.4-D was suitable for inducing PLB from leaf tissue. The best shoot formation was obsserved in MS medium supplemented with 10% coconut water and 2.0 mg l-1 BAP. In vitro rooting was best suited on MS medium supplemented with 1.0 mg l-1 NAA. These results could be the basis for building an in vitro propagation process for Mokara orchid which could applied in the production of this valuable orchid variety.Mokara là giống lan lai có nguồn gốc di truyền từ ba chi khác nhau Arachnis, Ascocentrum và Vanda. Đây là một trong những loại hoa lan cắt cành quan trọng đang được trồng rộng rãi ở nhiều nước như Singapore, Indonesia, Thái Lan… và Việt Nam. Nghiên cứu này hướng đến nhân giống in vitro lan Mokara từ mô lá thông qua tạo PLB. Kết quả nghiên cứu cho thấy, môi trường MS có bổ sung 2,0 mg/l 2,4-D thích hợp với việc cảm ứng tạo PLB từ mô lá. Sự tạo cụm chồi tốt nhất ở môi trường MS có bổ sung 10% nước dừa và 2,0 mg/l BAP. Sự tạo rễ lan Mokara thích hợp nhất ở môi trường MS có bổ sung 1,0 mg/l NAA. Kết quả nghiên cứu này bước đầu góp phần xây dựng quy trình nhân giống in vitro lan Mokara ứng dụng trong sản xuất giống lan có giá trị này.

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