ĐỊNH DANH BA LOÀI, Paris fargesii, Paris polyphylla, Paris vietnamensis THU TẠI VIỆT NAM VÀ SUY LUẬN SỰ PHÁT SINH LOÀI Ở CHI Paris

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Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2020

Mô tả vật lý: tr.395-402

Bộ sưu tập: Báo, Tạp chí

ID: 302511

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Các loài thuộc chi Paris (Bảy lá một hoa) là loại dược liệu quý, chứa nhiều loại dược chất có tác dụng cân bằng nội môi, kháng khuẩn, kháng virus, chống viêm và ức chế tế bào ung thư. Tuy nhiên, do khai thác quá mức để sản xuất dược phẩm đã phá hủy quần thể tự nhiên, làm cho số cá thể trong mỗi loài trong chi này còn rất ít, do đó, các loài Bảy lá một hoa cần được thực hiện các biện pháp bảo tồn khẩn cấp. Chính vì vậy, việc thu thập, định danh loài và phân tích phát sinh loài làm cơ sở xây dựng các biện pháp bảo tồn, phát triển các loài thuộc chi Paris ở Việt nam. Trong nghiên cứu này, ba loài thuộc chi Paris (Paris fargesii, Paris polyphylla var chinensis, Paris vietnamensis) thu tại Lai Châu, Lạng Sơn và Thanh Hóa (Việt Nam) được định danh dựa trên phương pháp hình thái so sánh và mã vạch DNA với trình tự vùng ITS và đoạn gen matK. Cây phát sinh loài được thiết lập dựa trên trình tự nucleotide của vùng ITS và đoạn gen matK phân lập từ ba loài P. fargesii, P. polyphylla var chinensis, P. vietnamensis ở Việt Nam và 21 loài thuộc chi Paris có trình tự ITS và matK trên GenBank theo phương pháp Maximum Likelihood đã cho thấy kết quả bước đầu về sự tiến hóa các loài thuộc chi Paris.Species of the genus Paris are precious medicinal herbs, which consist of many kinds of pharmaceutical substances with homeostasis effect, antibacterial, antiviral, anti-inflammatory and detoxification activities as well as inhibition of cancer cells. However, due to overexploitation of these species to produce pharmaceuticals, the number of individuals in each species of the Paris genus declined dramatically, which is why it is necessary to carry out emergency conservation measures for the Paris species. Therefore, the collection, identification and phylogenetic analysis of Paris species are a basis for conduction of conservation and development measures for species of the genus Paris in Vietnam. In this study, three species of the genus Paris (Paris fargesii, Paris polyphylla var chinensis, Paris vietnamensis) collected in Lai Chau, Lang Son and Thanh Hoa (Vietnam) were identified based on comparative morphology and DNA barcode including ITS and matK. Phylogenetic tree was established based on nucleotide sequences of ITS region and matK gene isolated from three Paris species of P. fargesii, P. polyphylla var chinensis, P. vietnamensis in Vietnam and ITS and matK sequences of 21 Paris species on GenBank using Maximum Likelihood method has shown initial results of evolution of Paris species.

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