ĐẶC ĐIỂM TRÌNH TỰ NUCEOTIDE CỦA CHỈ THỊ PHÂN TỬ SSR TRONG GEN MÃ HÓA SUCROSE SYNTHASE PHÂN LẬP TỪ GIỐNG CHÈ TRUNG DU XANH VÀ TRUNG DU TÍM

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Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2020

Mô tả vật lý: tr.461-465

Bộ sưu tập: Báo, Tạp chí

ID: 302727

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cDNA/EST - derived SSR markers (single sequence repeat-SSR) has the advantage of reducing the cost and time of directing the marker because cDNA/EST fragments are directly related to functional genes. In this study, we conducted an analysis of the nucleotide sequence of SSR marker of the gene encoding sucrose synthase - the enzyme involved in sucrose synthesis as a basis for molecular markers applied in tea breeding. Motif TTGTT belongs to 3’ UTR (untranslated region) of the gene encoding sucrose synthase was repeated in two samples of the interrupted group by a few bases that are not motif structure, the motif sequence obtained in green and purple Trung du tea, respectively: (TTGTT) 4 GTT (TTGTT) 4
(TTGTT) 6 GTT (TTGTT) 3
(TTGTT) 5 GTT (TTGTT). Comparing the TTGTT sequence of the sucrose synthase encoding gene with the published sequences shows that the TTGTT mottif has a conservative in varieties and a high polymorphism among the analyzed tea varieties.Chỉ thị SSR (single sequence repeat-SSR) có nguồn gốc từ cDNA/EST có ưu điểm giảm chi phí và thời gian xây dựng chỉ thị do các đoạn cDNA/EST liên quan trực tiếp đến các gen chức năng Trong nghiên cứu này, chúng tôi tiến hành phân lập, xác định và phân tích trình tự nucleotide chỉ thị SSR của gen mã hóa sucrose synthase - enzyme tham gia vào quá trình tổng hợp sucrose làm cơ sở nghiên cứu chỉ thị phân tử ứng dụng trong chọn tạo giống chè. Motif TTGTT thuộc đầu 3’UTR (Untranslated region) của gen mã hóa sucrose synthase được lặp lại trong hai mẫu nghiên cứu thuộc nhóm gián đoạn bởi một vài base không thuộc cấu trúc motif, trình tự motif thu được tương ứng ở chè Trung du xanh và Trung du tím lần lượt là: (TTGTT)4 GTT (TTGTT)4
(TTGTT)6 GTT (TTGTT)3
(TTGTT)5 GTT (TTGTT). So sánh trình tự motif TTGTT của gen mã hóa sucrose synthase với các trình tự đã công bố cho thấy, mottif TTGTT có thể có tính bảo thủ trong giống và tính đa hình cao giữa các giống chè phân tích.

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