Đánh giá hoạt tính kháng viêm, kháng khuẩn và ức chế enzyme α-glucosidase in vitro của nấm Ophiocordyceps sinensis giàu selen.

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Tác giả: Dang Khoa Nguyen Hoang, Ke Suong Ngo, Minh Hiep Dinh, Quoc Phong Le, Tai Hoang Nguyen, Quyen Dang Tu

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ Việt Nam - B, 2020

Mô tả vật lý: tr.19

Bộ sưu tập: Báo, Tạp chí

ID: 308536

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Ophiocordyceps sinensis (O. sinensis) - commonly known as Dong Chong Xia Cao, is a valuable medicinal fungus containing many bioactive compounds such as cordycepin, D-mannitol, polysaccharide, etc. While selenium is an essential trace element for humans, it is the main component of some enzymes such as glutathione peroxidase, thioredoxin reductase, and deiodinase which play an important role in scavenging free radicals, enhancing reproductive and muscle function, and preventing cancer. Several studies have shown that inorganic selenium added to O. sinensis culture can be converted into a beneficial organic form, which increases the value for the fungus. In this study, extracts from Se-enriched O. sinensis were evaluated some biological activities such as anti-inflammatory, antibacterial, and α-glucosidase inhibition. Results showed that Bu extract (extracted by n-butanol), crude extract, and EtOAc extract (extracted by ethyl acetate) can resist to S. sonnei at a concentration of 1000 µg/ml. Besides, the anti-inflammatory activity was stronger in crude extract, PE extract, and EtOAc extracts with IC50 values were 195.58±7.89
64.19±0.22
469.27±16.57 (µg/ml), respectively. However, all extracts had no ability to inhibit α-glucosidase. This study exhibited that extracts from Se-enriched O. sinensis have an admirable anti-inflammatory activity, which is a prerequisite for in vivo model research.Ophiocordyceps sinensis (O. sinensis) - đông trùng hạ thảo, là một loại nấm dược liệu quý chứa nhiều hợp chất có hoạt tính sinh học như cordycepin, D-mannitol, polysaccharide... Trong đó, đặc biệt có selen - một nguyên tố vi lượng cần thiết cho con người, là thành phần cấu tạo chính của một số enzyme như glutathione peroxidase, thioredoxin reductase và deiodinase. Các enzyme này đóng vai trò quan trọng trong việc chống các gốc tự do, tăng cường chức năng sinh sản, chức năng cơ và ngăn ngừa ung thư. Nghiên cứu này tiến hành chiết cao và khảo sát các hoạt tính sinh học như kháng viêm, kháng khuẩn và ức chế enzyme α-glucosidase của các cao chiết từ sinh khối O. sinensis giàu selen. Kết quả cho thấy, cao Bu (cao chiết bằng n-butanol)
cao tổng và cao EtOAc (chiết bằng ethyl acetate) có khả năng kháng khuẩn S. sonnei ở nồng độ 1000 µg/ml. Đồng thời, hoạt tính kháng viêm thể hiện tốt ở cao tổng, cao petrolium ether (cao PE) và cao EtOAc với IC50 lần lượt là 195,58±7,89
64,19±0,22
469,27±16,57 (µg/ml). Tuy nhiên, các cao chiết không có khả năng ức chế enzyme α-glucosidase. Nghiên cứu này cho thấy, các cao chiết từ sinh khối O. sinensis giàu selen có hoạt tính kháng viêm rất tốt, làm tiền đề cho các nghiên cứu trên mô hình in vivo.

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