In the present study, silver and copper nanoparticles were synthesized by chemical microwave polyolmethod, where AgNO3 and CuC2O4 were taken as metal precursors, glycerol as a reducing agent.TEM image of the obtained product showed the average particle size of 50 to 60 nm. Antifungal activityof the synthesized Ag/Cu nanomaterial was studied against the crop pathogenic fungi Colletotrichum spand Neoscytalidium dimidiatum isolated from infected dragon plant in Long An province by Institute ofBiotechnology, VAST. Assessment of fungicidal activity of the Ag/Cu nanomaterial showed that thisproduct exhibited strong antifungal activity towards dragon pathogenic fungi. At highest nanosilverconcentration of the Ag/Cu nanoparticles (40/24 ppm) sclerotial germination of Colletotrichum sp wasalmost inhibited (after 7 days of inoculation the inhibition effect on sclerotial germination attained100%). For the case of Neoscytalidium dimidiatum, after 3 days at 40/24 ppm Ag/Cu NPsconcentration the inhibition effect on sclerotial germination attained 93.3%. Comparing antifungalactivity of total nano Ag/Cu’s concentration with specific activities of silver and copper NPs separatelywhich have the same concentration showed that the antifungal activity of Ag/Cu nanoparticles wasnoticeably higher, confirming the synergic effect of these metals. The obtained results suggested thatthe synthesized Ag/Cu nanomaterial acts as an effective antifungal agent. Thus, it could be used indeveloping novel antifungal agents for potential applications in agriculture.Keywords: dragon, antifungal activity, Colletotrichum sp, Neoscytalidium dimidiatum.