In this paper we announce the survey results of the optimum conditions to simultaneous determinationof vitamine B1, vitamine B6 and vitamine B12 by high performance liquid chromatography (HPLC)method and simultaneously quantify vitamine B1, vitamine B6 and vitamine B12 in milk and fresh milkDIELAC GROW PLUS, DIELAC ALPHA GOLD by HPLC method. The results showed that the errorwhen determining vitamine B1, vitamine B6 and vitamine B12 are less than 3% with the recovery ofvitamine B1 from 91.35% to 100.81%
of vitamine B6 from 90.446% to 99.12%, of vitamine B12 from92.38% to 100.68%.