XÁC ĐỊNH, PHÂN NHÓM VIRUS GÂY BỆNH VIÊM PHẾ QUẢN TRUYỀN NHIỄM TRÊN GÀ NĂM 2018 Ở ĐỒNG BẰNG SÔNG CỬU LONG

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Tác giả: Cảm Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2019

Mô tả vật lý: tr.5

Bộ sưu tập: Báo, Tạp chí

ID: 311845

Thêm vào giỏ Liên kết toàn văn

Tám chuỗi nucleotide của gen nucleoprotein (N) gồm 403 bp đã được thu nhận từ 7 mẫu bệnh phẩm IBV trên gà nuôi ở hai tỉnh Sóc Trăng và Vĩnh Long bằng PCR và giải trình tự. Trình tự nucleotide gen N của IBV được đưa vào sắp xếp so sánh, phân tích phả hệ (25 chủng) và tính toán khoảng cách di truyền (20 chủng). Kết quả nghiên cứu cho thấy giữa 8 chủng của Việt Nam, một số nhóm chủng có khoảng cách di truyền rất thấp, cụ thể: 0-0,2% giữa 3 chủng IBST3, IBST4, IBST9
0,5% giữa 2 chủng IBST5-1 và IBST5-2 và 0% giữa IBST14 và IBVL22. Phân tích phả hệ đã chia 25 chủng IBV thành 2 nhóm chính, bao gồm: Nhóm 1 tập hợp các mẫu của thế giới và 3 mẫu IBV phân lập năm 2018 tại Sóc Trăng (IBST4, IBST3, IBST9) với chủng vacxin 4-91 (KF377577) và 2 chủng (IBST5-1, IBST5-2) cùng với Mass41 (AY851295)
và nhóm 2 gồm các chủng có nguồn gốc hoàn toàn từ Trung Quốc và 2 chủng phân lập ở Vĩnh Long (IBVL17, IBVL22) và 1 chủng phân lập ở Sóc Trăng (IBST14) cùng với QX và LX4 (QX-like) của Trung Quốc. Có ít nhất 3 nhóm IBV đã xác định được là đang nhiễm trên đàn gà và có sự đa nhiễm trên cùng một cá thể, trong đó có nhóm 4-91, Mass (Mass41) và QX (LX4 hay QX-like) phân lập năm 2018 tại Sóc Trăng và Vĩnh Long.There were 8 nucleotide sequences of the nucleoprotein gene (N) including 403 bp collected from 7 IBV specimens on the chickens raising in Soc Trang and Vinh Long provinces identified by PCR and sequencing. The nucleotide sequences of gene N of IBV were arranged and compared. The pedigree of 25 strains was analyzed and genetic distance of 20 strains was calculated. The studied result showed that among 8 IBV strains isolated in Viet Nam, some strain groups presented a very low genetic distance, for example: 0-0.2% among 3 strains (IBST3, IBST4, IBST9), 0.5% between 2 strains (IBST5-1, IBST5-2) and 0% between 2 strains (IBST14 and IBVL22). Base on pedigree analysis, 25 IBV strains were divided into 2 groups, including: Group 1 consisted of all IBV strains in the world, 3 IBV strains isolated in 2018 at Soc Trang (IBST4, IBST3, IBST9), vaccine strain 4-91 (KF377577), and 2 strains (IBST5-1, IBST5-2) together with Mass 41 (AY851295). Group 2: consisted of all the Chinese IBV strains and 2 strains isolated in Vinh Long (IBVL17, IBVL22) and 1 strain isolated in Soc Trang (IBST14) together with QX and LX4 (QX-like) strains of China. At least, there were 3 IBV strains determined to be infected on the chicken herds, presenting co-infection in the same chicken. Of which, there were group 4-91, Mass (Mass41) and QX (LX4 or QX-like) isolated in 2018 in Soc Trang and Vinh Long.

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