SỰ HIỆN DIỆN CỦA VIBRIO PARAHAEMOLYTICUS 03::k8 TRONG MÔI TRƯỜNG NƯỚC NUÔI THỦY SẢN, HẢI SẢN TƯƠI SỐNG Ở ĐỒNG BẰNG SÔNG CỬU LONG

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Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2019

Mô tả vật lý: tr.38

Bộ sưu tập: Metadata

ID: 312100

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Vibrio parahaemolyticus is one of the important foodborne pathogens in human. Most of the infected cases relate to raw or undercook seafood. Among the serotypes of pathogenic V. parahaemolyticus, O3:K6 is the most important serotype because it was predominant serotype isolated from the patients in the worldwide. In this study, the raw seafood and water samples in the Mekong Delta were examined for prevalence of V. parahaemolyticus associated with foodborne illness in human. 204 raw seafood samples were collected in the retail markets, 16 clam samples were collected from the clam farms, 39 shrimp samples were collected from the intensive shrimp culture ponds, 30 water samples were collected from Mekong river, 22 water samples were collected from the clam farms and 42 water samples were collected from the intensive shrimp culture ponds, they were used as materials for this study.The studied results indicated that there were 10/172 (5.8%) mollusca samples positive with V. parahaemolyticus carrying tdh virulent gene
meanwhile, this pathogenic bacterium was not found in the shrimp samples and other seafood samples collected from the aquaculture farms and river water samples collected from the Mekong Delta. 10 V. parahaemolyticus isolates carrying tdh gene were analyzed and from that serotypes and pathogenic signs were identified. Of which, 4 strains belonged to serotype O3:K6 and all of 4 these strains were positive with the signs of causing the intestinal diseases in human. The other strains were negative to cause diseases. This studied result can be used for understanding microbiological risk of raw seafood in the Mekong DeltaVibrio parahaemolyticus là một trong những nhân tố quan trọng gây bệnh đường ruột trên người. Phần lớn các trường hợp nhiễm V. parahaemolyticus có liên quan đến ăn hải sản sống. Trong các type huyết thanh của V. parahaemolyticus phân lập từ bệnh nhân tiêu chảy, O3:K6 là type phổ biến nhất ở người nhiễm bệnh. Trong nghiên cứu này, mẫu hải sản tươi sống và mẫu nước nuôi nghêu, tôm ở đồng bằng sông Cửu Long (ĐBSCL) đã được kiểm tra để phát hiện V. parahaemolyticus mang gen độc lực gây bệnh đường ruột. 204 mẫu hải sản tươi sống đã được thu từ các cửa hàng bán lẻ, 16 mẫu nghêu đã được thu từ vùng nuôi nghêu và 39 mẫu tôm đã được thu từ ao nuôi tôm thâm canh cùng với 30 mẫu nước sông, 22 mẫu nước ở vùng nuôi nghêu và 42 mẫu nước ở ao nuôi tôm thâm canh cũng đã được thu để phục vụ cho nghiên cứu này.Kết quả nghiên cứu cho thấy có 10/172 (5,8%) mẫu nhuyễn thể ở cửa hàng bán lẻ dương tính với V. parahaemolyticus mang gen độc lực tdh. Tuy nhiên, chủng vi khuẩn này không được tìm thấy trong mẫu tôm thu ở cửa hàng bán lẻ, mẫu hải sản thu ở trại nuôi và các loại mẫu nước đã thu. 10 chủng V. parahaemolyticus mang gen tdh phân lập đã xác định được type huyết thanh và dấu hiệu gây bệnh. Trong đó có 4 chủng thuộc type O3:K6 và cả 4 chủng này đều dương tính với dấu hiệu gây bệnh đường ruột ở người. Còn các chủng khác thì âm tính với dấu hiệu gây bệnh. Nghiên cứu này cung cấp thêm thông tin giúp hiểu rõ hơn về mối nguy vi sinh trong thực phẩm hải sản tươi sống ở ĐBSCL.

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