PHÁT HIỆN PORCINE CIRCOVIRUS TYPE 3 VÀ MỘT SỐ MẦM BỆNH KHÁC TRONG CÁC CA BỆNH HÔ HẤP PHỨC HỢP

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Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2019

Mô tả vật lý: tr.24

Bộ sưu tập: Báo, Tạp chí

ID: 312375

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Mục tiêu của nghiên cứu này nhằm bước đầu thăm dò sự hiện diện của Porcine circovirus type 3 (PCV3), đặc trưng kiểu gen ORF2 và sự đồng nhiễm với một số mầm bệnh khác trong các ca bệnh hô hấp phức hợp (PRDC) trên heo sau cai sữa. Tỷ lệ phát hiện PCV2 trên heo bệnh hô hấp phức hợp là cao nhất (88,3%
53/60), tiếp đến là vi rút gây hội hứng rối loạn sinh sản và hô hấp - PRRSV (55,0%
33/60), Mycoplasma hyopneumonia - Mh (51,7%
31/60), Haemophilus parasuis - Hp (44,3%
27/60), dịch tả heo - CSFV (13,3%
8/60) và thấp nhất là PCV3 (6,67%
4/60). Các nhóm mầm bệnh nhiễm ghép cao với nhau trong các ca hô hấp phức hợp là PCV2 + PRRSV (48,3%), PCV2 + Mh (50,0%), PCV2 + Hp (38,3%) và PCV2 + Mh + Hp (23,3%). Với PCV3, cả 4/4 trường hợp phát hiện đều nhiễm ghép với PCV2, PRRSV, Mh hoặc Hp
trong đó 4/4 nhiễm ghép với PCV2, 2/4 nhiễm ghép với PRRSV và Mh, 1/4 nhiễm ghép với Hp và 0/4 nhiễm ghép với CSFV. Mức tương đồng của trình tự nucleotide giữa 4 chủng PCV3 là 98,2 đến 97,2 %, trong đó 2 chủng PCV3_AV và PCV3_BL nằm tách biệt với các chủng PCV3 còn lại và các chủng tham khảo trên cây sinh dòng. Kết quả nghiên cứu cho thấy có sự hiện diện PCV3 ở các ca bệnh PRDC và chúng bắt đầu có sự đa dạng lớn về kiểu gen, nên nghiên cứu tiếp theo nhằm xác định vai trò gây bệnh giữa các chủng khác kiểu gen là rất cần thiết.The aim of this study was to identify initially the presence of PCV3, characterize ORF2 characteristics and co-infection with other pathogens in porcine respiratory disease complex (PRDC) in the post-weaning piglets. The PCV2 detection rate in the PRDC pigs was highest (88.3%
53/60), followed by PRRSV (55.0%
33/60), Mh (51.7%
31/60), Hp (44.3%
27/60), CSFV (13.3%
8/60) and the lowest rate was PCV3 (6.67%
4/60). The co-infection groups of PRDC cases were PCV2 + PRRSV (48.3%), PCV2 + Mh (50.0%), PCV2 + Hp (38.3%) and PCV2 + Mh + Hp (23.3%). In term of PCV3, all 4/4 cases were co-infected with PCV2, PRRSV, Mh or Hp
of which 4/4 co-infected with PCV2, 2/4 co-infected with PRRSV and Mh and 1/4 co-infected with Hp and 0/4 co-infected with CSFV. The low nucleotide sequence similarity level among 4 isolates of PCV3 (98.2 to 97.2%) was determined, of which two isolates PCV3_AV and PCV3_BL were separately grouped from the remaining PCV3 isolates and reference strains in phylogenetic tree. The studied results showed that there was the presence of PCV3 in the PRDC cases and they began presenting a high diversity on genotype, therefore further research to identify the role of pathogenicity among the strains with different genotype will be very necessary.

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