Phát hiện và phân tích đặc điểm di truyền của Canine Kobuvirus trên chó nuôi tại Thành phố Hồ Chí Minh

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Tác giả:

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2019

Mô tả vật lý: tr.35

Bộ sưu tập: Báo, Tạp chí

ID: 312825

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Canine Kobuvirus (CKoV) is a pathogen relating to enteritis in the domestic dogs. Currently, prevalence of CKoV in dogs has been reported in many countries. However, information on CKoV in the domestic dogs is lack in Viet Nam at present. The objective of this study was to investigate and charaterize CKoV, circulating in Ho Chi Minh City, Viet Nam. A total of 80 fecal swab samples from dogs (30 samples from the diarrheic dogs, 50 samples from the healthy dogs) were detected for CKoV by RT-PCR technique. The studied result showed that the prevalence of CKoV in the diarrheic dogs was 10% higher than that in the healthy dogs (4%). However, there was no statistically significant differences in prevalence of CKoV from the diarrheic and health dogs (P>
0.05). The infection of CKoV with other enteritic viruses (CPV, CDV and MRV) was commonly found in the domestic dogs. It indicated that CKoV was a minor pathogen in enteritis in dogs. Genetic analysis showed that 5 detected CKoV strains presented high similarity level to Korean and British CKoV strains (95.8%-99.5%). This is the first report on CKoV infection in the domestic dogs in Ho Chi Minh City.Canine Kobuvirus (CKoV) là một trong những tác nhân có liên quan đến viêm đường ruột trên chó nuôi. Tỷ lệ lưu hành CKoV đã được báo cáo ở nhiều nước. Tuy nhiên, ở Việt Nam hiện tại thiếu thông tin về CKoV trên chó nuôi. Nghiên cứu này nhằm mục đích đánh giá sự lưu hành và xác định các đặc tính di truyền liên quan CKoV trên chó nuôi tại Thành phố Hồ Chí Minh. Kết quả kiểm tra 80 mẫu swab phân từ chó nuôi (30 chó tiêu chảy, 50 chó khỏe) bằng kỹ thuật RT-PCR, cho thấy tỷ lệ lưu hành của CKoV ở nhóm chó bệnh là 10%, cao hơn ở nhóm chó khỏe (4%). Tuy nhiên, sự khác biệt này không có ý nghĩa thống kê (P>
0,05). CKoV thường phát hiện nhiễm cùng với các virus gây bệnh viêm đường ruột khác (CPV, CDV, CCoV và MRV). Điều này cho thấy CKoV có thể là một tác nhân phụ trong các bệnh gây viêm ruột trên chó. Kết quả phân tích đặc tính di truyền cho thấy có 5 chủng CKoV phát hiện được trên chó nuôi ở Thành phố Hồ Chí Minh có mức tương đồng cao với các chủng CKoV từ Hàn Quốc, Anh (95,8%-99,5%). Đây là báo cáo về sự nhiễm CKoV đầu tiên trên chó nuôi tại Thành phố Hồ Chí Minh.

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