Đa hình rs32o trên gen Lipoprotein lipase (LPL) đã được báo cáo có liên quan đến triglycerid, cholesterol toàn phần, lipoprotein tỉ trọng cao, lipoprotein tỉ trọng thấp.The rs320 polymorphism of LPL gene has been reported to be associated with triglycerides, total cholesterol, HDL-C, and LDL-C. To analyze this polymorphism in Vienamese populations, the study aimed to optimize the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method with primers, thermal cycles, and restriction enzymewhich were selected and tested to identify opimal condition of PCR-RFLP method. The GG, TG, and TT genotypes by PCR - RFLP methods were confirmed by the sequencing method. Theexperimental results determined the annealing temperature of 57 oC for PCR, the minimum 3 units of HindIII for incubation with PCR product. In conclusion, this optimal PCR-RFLPmethod can be applied to genotype LPLrs320 in large populations to investigate the association between the rs320 polymorphism and dyslipidemia.