NGHIÊN CỨU SỰ PHÁT SINH HÌNH THÁI CỦA CÂY BÁ BỆNH (Eurycoma longifolia) TỪ CÁC NGUỒN MÔ KHÁC NHAU DƯỚI TÁC ĐỘNG CỦA CHẤT ĐIỀU HÒA SINH TRƯỞNG THỰC VẬT

0 Người đánh giá. Xếp hạng trung bình 0

Tác giả: Đăng Giáp Đỗ, Lệ Hoa Tiên Nguyễn, Thị Dược Nguyễn, Thị Huyền Trang Nguyễn, Trọng Tuấn Trần, Thị Hương Trịnh

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học Công nghệ và Thực phẩm (Tên mới: Tạp chí Khoa học Đại học Công Thương), 2020

Mô tả vật lý: tr.80

Bộ sưu tập: Metadata

ID: 313134

Thêm vào giỏ Liên kết toàn văn

Nghiên cứu này được thực hiện nhằm khảo sát ảnh hưởng của chất điều hoà sinh trưởng thực vật và loại mẫu cấy đến quá trình phát sinh hình thái ở cây bá bệnh. Kết quả thu được cho thấy, đối với mẫu cấy tử diệp, tỷ lệ hình thành mô sẹo và khối lượng tươi đạt cao nhất, lần lượtlà 96,3% và 258 mg khi nuôi cấy trên môi trường MS có bổ sung 3,0 mg/L NAA kết hợp với 0,5 mg/L BA
mô sẹo thu được có màu vàng, xốp. Đối với mẫu cấy lá non, tỷ lệ hình thành mô sẹo và khối lượng tươi đạt cao nhất, lần lượt là 100% và 99 mg khi nuôi cấy trên môi trường MS có bổ sung 0,5 mg/L NAA kết hợp với 0,5 mg/L BA
mô sẹo thu được có màu trắng, xốp. Đối với mẫu cấy cuống lá non, tỷ lệ hình thành mô sẹo và khối lượng tươi đạt cao nhất, lần lượt là 100% và 56 mg khi nuôi cấy trên môi trường MS có bổ sung 1,0 mg/L NAA kết hợp với 2,0 mg/L BA
mô sẹo thu được có màu trắng, xốp. Sự phát sinh hình thái mô sẹo của cả 3 loại mẫu trên môi trường nuôi cấy có bổ sung 6-benzylaminopurine (BA) kết hợp với thidiazuron (TDZ) cho hiệu quả thấp hơn so với khi sử dụng BA kết hợp với NAA. Mô sẹo thu được có hình thái và màu sắc khác nhau, đây sẽ là nguồn nguyên liệu phục vụ cho nhiều hướng nghiên cứu tiếp theo như: nuôi cấy phát sinh phôi, nuôi cấy huyền phù thu nhận hợp chất thứ cấp… Trong nghiên cứu phát sinh rễ bất định của mẫu lá in vitro, NAA thích hợp cho sự hình thành rễ bất định của Eurycoma longifolia. Kết quả nghiên cứu cho thấy tỷ lệ mẫu hình thành rễ, số rễ/mẫu và chiều rễ trung bình đạt được cao nhất, lần lượt là 80%, 2,5 rễ/mẫu, 23,8 mm, tỷ lệ mẫu tạo rễ 80,0% ở nghiệm thức sử dụng 1,0 mg/L NAA. Không có sự hình thành rễ ở mẫu lá ex vitro ở tất cả các nghiệm thức.Từ khoá: BA, cây bá bệnh, cuống lá, lá, mô sẹo, NAA, TDZ, tử diệp.This study was conducted to investigate the effect of plant growth regulators and the type of explants on morphogenesis in Eurycoma longifolia. The results showed that, for the cotyledon explants: the rate of callus formation and the fresh weight were the highest, 96.3% and 258 mg respectively when the explants were cultured on the MS medium supplemented with 3.0 mg/L NAA in combination with 0.5 mg/L BA, the calli were yellow and friable
for young leaf explants: the rate of callus formation and fresh weight reached the highest, 100% and 99 mg respectively when the explants were cultured on the MS medium supplemented with 0.5 mg/L NAA in combination with 0.5 mg/L BA, the calli were white and friable
for young petiole explants, the rate of callus formation and fresh weight were the highest 100% and 56 mg respectively when the explants were cultured on the MS medium supplemented with 1.0 mg/L NAA in combination with 2.0 mg/L BA, calli were white and friable. The callogenesis time of the petiole explants occurred earlier than callogenesis time of leaf and cotyledons explants. The callus formation rate of treatment using BA combined with TDZ was lower than treatment using BA combined with NAA. The results of this study show that the collected calli have different morphology and colors. This will be a diverse source of materials to serve many different research directions such as embryogenesis, cell suspension culture to receive secondary compounds... In the study of adventious rooting of in vitro leaf explants, NAA is suitable for the formation of adventious roots of Eurycoma longifolia. The research results showed that the average rooting ratio, number of roots/explants and average root length reached the highest, 80%, 2.5 roots/explants, 23.8 mm respectively in the treatment using 1.0 mg/L NAA. There was no root formation in ex vitro leaves explants in all treatments.Keywords: BA, callus, cotyledon, Eurycoma longifolia, leaves, NAA, petiole, TDZ.

1. Đọc trực tuyến

1. Đọc trực tuyến

Tạo bộ sưu tập với mã QR