The Amazon Sustainable Landscapes Program (ASL) is an initiative funded by the Global Environment Facility (GEF) that seeks to improve integrated landscape management and ecosystem conservation in priority areas of the Amazon in Bolivia, Brazil, Colombia, Ecuador, Guyana, Peru and Suriname. The national level environmental agencies of each country lead the implementation of the respective projects, and multiple public and private institutions co-execute the projects, along with civil society and community organizations. The World Bank (WB) is the lead agency for the Program and, together with WWF, CAF, FAO, UNIDO, IFAD and UNDP, supports countries in the preparation and monitoring of national projects. The ASL also includes a regional project executed by the WB that promotes coordination and exchange of experiences. The ASL, particularly its regional coordination project, works to establish and strengthen networks of people and institutions to share ideas, lessons learned and innovations, accelerate learning, and generate/systematize/socialize knowledge at various levels and for diverse audiences. The ASL Annual Conference is designed to be the largest gathering of the ASL community. The Fourth ASL Conference was held from November 17-18, 2021. Considering the current mobility restrictions due to COVID-19, this conference was held virtually.