Nghiên cứu chức năng của Botrytis Susceptible1 Interactor (BOI) trong điều khiển sự ra hoa ở cây Arabidopsis

0 Người đánh giá. Xếp hạng trung bình 0

Tác giả: Thị Khoa Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Nguyễn Tất Thành, 2020

Mô tả vật lý: tr.32

Bộ sưu tập: Metadata

ID: 324718

Thêm vào giỏ Liên kết toàn văn

A Really Interesting New Gene (RING) finger protein BOTRYTIS SUSCEPTIBLE1 INTERACTOR (BOI) represses the expression of the Flowering Locus T gene (FT) in Arabidopsis thaliana, hence the inhibition of flowering in long days. To investigate the molecular function of BOI, we studied the interaction of BOI with a histone deacetylase complex (HDAC) since this complex also acts as a repressive regulator of FT expression. Here, we report that BOI directly interacted with HISTONE DEACETYLASE 6 (HDA6), an enzyme subunit of HDAC in yeast and in vitro. A yeast bihybrid assay showed that yeast cells containing both BOI and HDA6 were able to grow on a selective medium without tryptophan, leucine, adenine và histidine, indicating a direct interaction of BOI and HDA6 in yeast. In addition, BOI also bound to gluthathion S-transferase (GST)-HDA6 but not to GST in an in vitro pull-down assay. These results suggest BOI might interact with HDAC to inhibit the expression of FT in Arabidopsis. Further in vivo studies are needed to uncover this molecular mechanism.Botrytis Susceptible1 Interactor (BOI) là một Really Interesting New Gen (RING) finger protein ức chế sự ra hoa bằng cách giảm sự biểu hiện của gen Flowering Locus T (FT), một yếu tố quyết định sự ra hoa ở cây Arabidopsis. Để làm rõ cơ chế phân tử BOI, chúng tôi tìm hiểu khả năng tương tác của BOI với protein trong phức hệ Histone Deacetylase Complex (HDAC) vì phức hệ này cũng có vai trò ức chế sự biểu hiện của FT. Nghiên cứu đã chứng minh được tương tác của BOI với Histone Deacetylase 6 (HDA6) - một protein thành phần trong HDAC, trên nấm men và trên in vitro. Kết quả lai đôi ở nấm men (yeast two-hybrid assay) cho thấy chỉ có tế bào nấm men chứa BOI và HDA6 mới sống được môi trường chọn lọc không chứa tryptophan, leucine, adenine và histidine. Điều này chứng tỏ tương tác giữa BOI và HDA6 là tương tác protein trực tiếp trên nấm men. Ngoài ra, phương pháp tương tác protein in vitro (in vitro pull-down assay) cũng cho thấy BOI tương tác đặc hiệu với HDA6 nhưng không tương tác với glutathion-S-transferase (GST). Kết quả này gợi ý BOI có thể ức chế FT thông qua HDAC. Cần có các nghiên cứu sâu hơn để chứng minh mối liên hệ giữa BOI và HADC trong ức chế sự biểu hiện của FT ở cây Arabidopsis

1. Đọc trực tuyến

1. Đọc trực tuyến

Tạo bộ sưu tập với mã QR