CYTOTOXIC AND IMMUNOSUPPRESSIVE ACTIVITIES OF METHYL CAFFEATE ISOLATED FROM BALANOPHORA LAXIFLORA

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Tác giả: Thi Cham Ba, Dang Ngoc Quang, Do Thi Thao, Domenico V Delfino, Thi Hong Nhung Le, Phi Hung Nguyen, Quyet Tien Nguyen, Thanh Tam Nguyen, Thi Hoang Anh Nguyen, Thi Thu Hoa Nguyen, Thi Thuy Linh Nguyen, Duc Dai Tran, Khac Vu Tran, Thi Thuy Trinh

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học & Công nghệ - Trường Đại học Công nghiệp Hà Nội, 2020

Mô tả vật lý: tr.114

Bộ sưu tập: Metadata

ID: 325373

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Methyl caffeat, một trong các chất chính, được phân lập bằng phương pháp sắc ký cột từ loài Ngọc cẩu (Balanophora laxiflora) mọc ở Tuyên Quang. Methyl caffeatđược đánh giá hoạt tính gây độc tế bào đối với năm dòng tế bào ung thư người: A549, T24, Huh-7, 8505c và SNU-1. Kết quả cho thấy methyl caffeat thể hiện hoạt tínhgây độc tế bào trên tất cả các dòng tế bào được thử nghiệm (A549, T24, Huh-7, 8505 và SNU-1) với giá trị IC50 trong khoảng từ 28,83µg/mL đến 50,19µg/mL. Ngoài ra,đây là lần đầu tiên khả năng ức chế miễn dịch của methyl caffeat thông qua ức chế biểu hiện interleukin (IL-2) trong tế bào đại thực bào (RAW264.7) được đánh giá.Kết quả cho thấy methyl caffeat có tác dụng ức chế mạnh sự bài tiết cytokine IL-2 trên tế bào RAW264.7 ở nồng độ 100µg/mL (P <
0,01). Những kết quả này cho thấymethyl caffeat có thể có tác dụng ức chế miễn dịch in vitro.Methyl caffeate, a major component, was isolated by column chromatography from Balanophora laxiflora growing in Tuyen Quang. The cytotoxic activity ofmethyl caffeate has been evaluated using A549, T24, Huh-7, 8505c and SNU-1 cell lines. Results showed that methyl caffeate exhibited cytotoxic activity against allfive tested human cancer cell lines (A549, T24, Huh-7, 8505 and SNU-1) with the IC50 values ranging from 28.83g/mL to 50.19g/mL. In addition, theimmunosuppressive capacity of methyl caffeate through inhibition of IL-2 expression in macrophage cells (RAW264.7) was assessed for the first time. The resultsexhibited that methyl caffeate had a strong inhibitory effect on IL-2 cytokine secretion on RAW264.7 cells at a concentration of 100µg/mL (P <
0.01). These resultssuggest that methyl caffeate could possess immunosuppressive effects in vitro.

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