A study was conducted to evaluate the uptake and accumulation of waterborne copper (Cu), lead (Pb) and cadmium (Cd) in flesh tissue of Tilapia (Oreochromis niloticus). Single breed fingerlings (7.8 ± 1.3 g) of Tilapia were obtained from Aquaculture No.1 (Bac Ninh, Viet Nam). Four groups of fish (40 to 45 each) were maintained in 100 liters of water in glass tanks. Tilapia were treated with the different concentrations of Cu, Pb (0, 0.02, 0.05, 0.2 mg/l) and Cd (0, 0.005, 0.01, 0.05 mg/l) for 60 days. Fish sampling was done on day zero and every 15 days. The copper, lead and cadmium concentration in flesh tissue in the experimental tanks increased with increasing exposure time. Statistical analyses indicated Cu, Pb and Cd levels in all treatment samples were significantly different from control (p<
0,01), but they did not differ between samples (p >
0.05). When compared to the day zero values, the levels of accumulation of cadmium at 60 days was highest.