NGHIÊN CỨU MỘT SỐ CHỈ TIÊU QUANG HỢP VÀ SINH TRƯỞNG CỦA CÂY CỎ NGỌT (Stevia rebaudiana BERTONI) NUÔI CẤY IN VITRO TRONG HỆ THỐNG CHIẾU SÁNG ĐƠN SẮC VÀ GIAI ĐOẠN NHÀ LƯỚI

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Tác giả: Thị Mai Hoa Điêu, Thị Thúy Hường Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học - Trường Đại học Sư phạm Hà Nội: Khoa học Tự nhiên, 2020

Mô tả vật lý: tr.137

Bộ sưu tập: Metadata

ID: 326652

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Cây cỏ ngọt (Stevia rebaudiana Bertoni) được xem là là loại cây dược liệu. Nghiên cứu này nhằm mục đích đánh giá khả năng sinh trưởng, tích lũy chất khô trong quang hợp của cây cỏ ngọt nuôi cấy trong điều kiện chiếu sáng có tỉ lệ thành phần quang phổ khác nhau, từ đó tìm ra tỉ lệ chiếu sáng phù hợp nhất. Vật liệu khởi đầu là những đoạn thân cây cỏ ngọt đem nuôi cấy trong môi trường tạo chồi gồm: MS cơ bản bổ sung 20 g\Lsucrose
8 g\Lagar
0,15 mg\LBAP, 0,2 mg\LNAA, nuôi cấy trong 4 tuần dưới hệ thống giàn đèn LED với các tỉ lệ ánh sáng màu khác nhau theo các công thức sau: 40% đỏ\20% xanh\40% cam (CT1), 20% đỏ\20% xanh\60% vàng (CT2), 72% đỏ\14% xanh\14% trắng (CT3) và 80% đỏ\20% xanh (CT4) và đối chứng là công thức chiếu đèn huỳnh quang (ĐC). Kết quả ở công thức CT4 cho tỉ lệ tạo chồi là 100%, chiều cao trung bình 48,5 mm\chồi và chất lượng chồi tốt nhất. Môi trường tạo rễ gồm: MS cơ bản bổ sung 15 g sucrose, 8 g\Lagar, 0,2 mg\LNAA trong 4 tuần. Kết quả cũng cho thấy, công thức chiếu sáng CT4 thích hợp nhất cho quang hợp và sinh trưởng của cây cỏ ngọt nuôi cấy giai đoạn tạo rễ. Những cây con từ hệ thống chiếu sáng này khi được trồng trong nhà lưới có khả năng sống sót, sinh trưởng tốt hơn cây từ các điều kiện chiếu sáng còn lại.This study aims to evaluate the growth and dry matter accumulation of stevia grown in lighting conditions with different spectral ratios, thereby finding the most suitable lighting rate. Materials are stevia stalks cultured in shoot medium, including: MS base supplemented with 20 g\Lsucrose
8 g\Lagar
0.15 mg\LBAP, 0.2 mg\LNAA, cultured for 4 weeks under a LED system with different color light ratios, using the following formulas: 40% red + 20% blue + 40% orange (CT1), 20% red + 20% blue + 60% yellow (CT2), 72% red + 14% blue + 14% white (CT3) and 80% red + 20% blue (CT4) and the control formula was the formula of fluorescent lamp irradiation (DC). Formula CT4 gave a result of 100% shoot formation, the average height of shoots was 48.5 mm\shoot and the shoots were the best in quality. Rooting medium included: MS basal supplemented with 15 g sucrose, 8 g\Lagar, 0.2 mg\LNAA for 4 weeks. The results also showed that the CT4 lighting formula was the most suitable for the photosynthesis and growth of cultured stevia in the rooting stage. The seedlings from this lighting system when grown in the net house were able to survive and grow better than the plants from the other lighting conditions.

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