XÁC ĐỊNH MỘT SỐ ĐẶC ĐIỂM CẤU TRÚC, VỊ TRÍ VÀ PHÂN NHÓM CỦA CÁC PROTEIN VẬN CHUYỂN ĐƯỜNG SUCROSE HỌ SWEET Ở CÂY DIÊM MẠCH (CHENOPODIUM QUINOA) BẰNG CÁC CÔNG CỤ TIN SINH HỌC

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Tác giả: Đức Hà Chu, Sông Thao Nguyễn, Phương Thu Phạm, Thị Thanh Huyền Trần

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học - Trường Đại học Sư phạm Hà Nội: Khoa học Tự nhiên, 2020

Mô tả vật lý: tr.188

Bộ sưu tập: Metadata

ID: 326705

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In this study, a total of 29 members of the CqSWEET family in quinoa (Chenopodium quinoa) has been comprehensively investigated by various bioinformatics methods. As the result, we indicated that the CqSWEET proteins were varied from 161 - 428 amino acid residues in size (17,7 - 47,7 kDa in weight). The isoelectric point of these proteins ranged from acid (4.9) to base (9,7), while the majority of the CqSWEET proteins are stable in the test tube. Our analyses also revealed that whole members of the CqSWEET family are hydrophobic, and a large amount of the CqSWEET proteins may localize in the secretory pathways. According to the unrooted phylogenetic tree, the CqSWEET proteins could be clearly classified into 3 distinct groups, as similar as in other higher plant species. Interestingly, the majority of the CqSWEET proteins contained 6 or 7 transmembrane helices. Our results also showed that a total of 4 conserved motifs has occurred in the majority of the CqSWEET family. Take together, our study could provide critical data for further functional characterization of the CaSWEET candidate genes related to the regulation of the growth and development in quinoa plants.Trong nghiên cứu này, 29 thành viên của nhóm protein CqSWEET trên cây diêm mạch (Chenopodium quinoa) đã được phân tích bằng các công cụ tin sinh học một cách toàn diện. Theo đó, các protein CqSWEET có kích thước đạt từ 161 - 428 axít amin (17,7 - 47,7 kDa). Giá trị điểm đẳng điện của các protein CqSWEET dao động từ ngưỡng axít (4,9) đến bazơ (9,7), trong khi đa số thành viên của nhóm CqSWEET có tính ổn định trong ống nghiệm. Phân tích cho thấy nhóm CqSWEET có tính kỵ nước và rất nhiều protein có thể cư trú ở hệ thống bao gói. Dựa theo sơ đồ hình cây, các protein CqSWEET được phân loại thành 3 nhóm, tương tự như ở các loài thực vật khác. Phân tích cấu trúc cho thấy phần lớn các protein CqSWEET đều mang 6 hoặc 7 cấu trúc xoắn xuyên màng. Nghiên cứu đã ghi nhận 4 motif bảo thủ xuất hiện ở hầu hết các thành viên của nhóm CqSWEET. Kết quả của nghiên cứu này đã cung cấp những dẫn liệu quan trọng cho việc phân tích chức năng của gen CqSWEET ứng viên liên quan đến điều hòa sinh trưởng và phát triển của cây diêm mạch.

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