Ứng dụng chỉ thị phân tử microsatellite trong truy xuất phả hệ quần đàn cá tra (Pangasianodon hypophthalmus) chọn giống

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Tác giả: Hoàng Khôi Nguyên Lê, Hoàng Thông Nguyễn, Hồng Lộc Nguyễn, Văn Sáng Nguyễn, Thị Phương Dung Trần

Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Khoa học và Công nghệ Việt Nam - B, 2022

Mô tả vật lý: tr.48

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ID: 327871

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Nghiên cứu sử dụng bộ chỉ thị gồm 10 microsatellite để truy xuất phả hệ quần đàn cá tra chọn giống thế hệ đầu tiên. Kết quả cho thấy: (1) Khi phân tích 50 mẫu cá tra bố mẹ G0 và 50 mẫu cá con G1 thì hiệu suất PCR đạt 98-100% và số lượng alen (NA) dao động 5-14 mỗi locus. Hệ số đa dạng di truyền (PIC) trung bình của các microsatellite trên G0 và G1 lần lượt là 0,71 và 0,67, trung bình tỷ lệ dị hợp tử quan sát (HO) và tỷ lệ dị hợp tử mong đợi (HE) trên tất cả các locus lần lượt là 0,73, 0,76, 0,73, 0,71. 10 microsatellite khảo sát đều ổn định và đa hình phù hợp với việc truy xuất phả hệ
(2) Sau khi sàng lọc các chỉ thị và truy xuất phả hệ trên 50 gia đình cá chọn giống cho thấy bộ chỉ thị với 9 microsatellite (loại trừ Pahy-02) truy xuất phả hệ cao, đạt được truy xuất bố mẹ chính xác 93,4%, trong đó truy xuất các gia đình con bố không có half-sib đạt 93,0% và các gia đình con bố có half-sib đạt 94,0%. Qua các kết quả cho thấy, bộ chỉ thị với 9 microsatellite có thể ứng dụng để truy xuất phả hệ trong các chương trình chọn giống trên cá tra.The research used ten newly developed microsatellites markers set which were assigned offsprings to their parents on the first generation of selection on striped catfish. The result shows: (1) When analysing 50 samples of parents (G0) and 50 samples of offsprings (G1), the PCR ratio was from 98-100%, the allele number (NA) ranged from 5-14 per locus. The average Polymorphic Information Content (PIC) of microsatellites on G0 and G1 were 0.71 and 0.67, respectively. The average observed heterozygosity (HO) and expected heterozygosity (HE) all over loci were 0.73, 0.76, and 0.73, 0.71, respectively. Ten microsatellites are stable and polymorphic that are suitable for parentage analysis. (2) After makers screening, parentage analysis on 50 selective families, the initial set of nine microsatellites (excluded Pahy- 02) for parentage analysis was effective with 93.4% of offspring allocated unambiguously to their parental pairs, of which families with the father not having half- sib, 93.0% of offspring was allocated unambiguously to their parental pairs and families with the father having half-sib, 94.0% of offspring allocated unambiguously to their parental pairs. The results show that the maker set with nine microsatellites can be used in parentage analysis for the breeding program on striped catfish.

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